从生牛奶和生牛奶奶酪中分离出的新物种——粗糙双歧杆菌（Bifidobacterium crudilactis sp. nov.）的描述。
Description of a new species, Bifidobacterium crudilactis sp. nov., isolated from raw milk and raw milk cheeses.
作者信息
Delcenserie V, Gavini F, Beerens H, Tresse O, Franssen C, Daube G
机构信息
Faculty of Veterinary Medicine, Food Sciences Department, University of Liege, Sart Tilman, B43b, B-4000 Liege, Belgium.
出版信息
Syst Appl Microbiol. 2007 Jul;30(5):381-9. doi: 10.1016/j.syapm.2007.01.004. Epub 2007 Feb 23.
A new Bifidobacterium species is described based on the study of ten Gram-positive strains with fructose-6-phosphate phosphoketolase activity. They are part of a phenotypic group comprising 141 strains isolated from raw milk and raw milk cheeses in French raw milk cheese factories. This group was separated by a numerical analysis based on API 50CH, API 32A tests and growth at 46 degrees C. A strong similarity of 16S rRNA sequences (99.8%) was shown between strain FR62/b/3(T) and Bifidobacterium psychraerophilum LMG 21775(T). However, low DNA-DNA relatedness was observed between their DNAs (31%). The new isolates are able to grow at low temperatures (all ten strains up to 5 degrees C) and strain FR62/b/3(T) grows under aerobic conditions, as does B. psychraerophilum. However, contrary to B. psychraerophilum, they do not ferment L-arabinose, D-xylose, arbutin or melezitose, but they do acidify lactose. The DNA G+C content of FR62/b/3(T) is 56.4mol%. Therefore, the name Bifidobacterium crudilactis sp. nov. is proposed, with its type strain being FR62/b/3(T) (=LMG 23609(T)=CNCM I-3342(T)).
基于对10株具有6-磷酸果糖磷酸酮醇酶活性的革兰氏阳性菌株的研究，描述了一个新的双歧杆菌物种。它们是一个表型组的一部分，该表型组包含从法国生牛奶奶酪工厂的生牛奶和生牛奶奶酪中分离出的141株菌株。通过基于API 50CH、API 32A测试以及在46℃下生长情况的数值分析，将该组进行了区分。菌株FR62/b/3(T)与嗜冷双歧杆菌LMG 21775(T)之间显示出16S rRNA序列的高度相似性（99.8%）。然而，它们的DNA之间的DNA-DNA相关性较低（31%）。新分离株能够在低温下生长（所有10株菌株均可在5℃下生长），并且菌株FR62/b/3(T)能够在有氧条件下生长，嗜冷双歧杆菌也是如此。然而，与嗜冷双歧杆菌不同的是，它们不发酵L-阿拉伯糖、D-木糖、熊果苷或松三糖，但它们能使乳糖酸化。FR62/b/3(T)的DNA G+C含量为56.4mol%。因此，提议将其命名为粗糙乳糖双歧杆菌（Bifidobacterium crudilactis sp. nov.），其模式菌株为FR62/b/3(T)（=LMG 23609(T)=CNCM I-3342(T)）。
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