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来自中国的一种新型马铃薯Y病毒重组分离株的全基因组分析

Complete genome analysis of a novel recombinant isolate of potato virus Y from China.

作者信息

Gao Fangluan, Chang Fei, Shen Jianguo, Shi Fengyang, Xie Lianhui, Zhan Jiasui

机构信息

Fujian Key Lab of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, P. R. China.

出版信息

Arch Virol. 2014 Dec;159(12):3439-42. doi: 10.1007/s00705-014-2184-2. Epub 2014 Aug 5.

Abstract

The complete sequence of GF_YL20, a potato virus Y (PVY) isolate from China, encodes a polyprotein of 3,061 amino acids. Sequence analysis indicates that GF_YL20 has a genomic structure different from previously reported PVY strains. It shares 99 % nucleotide sequence identity with PB209 (PVY(N:O)) except in VPg, but more than 97 % nucleotide sequence identity with the VPg of Mont (PVY(N)), PB312 (PVY(NTN)) and HN2 (SYR-I). Phylogenetic analysis indicates that GF_YL20 is a novel N:O recombinant with three recombination breakpoints.

摘要

GF_YL20是一种来自中国的马铃薯Y病毒(PVY)分离株,其完整序列编码一个由3061个氨基酸组成的多聚蛋白。序列分析表明,GF_YL20的基因组结构与先前报道的PVY株系不同。除VPg外,它与PB209(PVY(N:O))的核苷酸序列同一性为99%,但与Mont(PVY(N))、PB312(PVY(NTN))和HN2(SYR-I)的VPg核苷酸序列同一性超过97%。系统发育分析表明,GF_YL20是一种具有三个重组断点的新型N:O重组体。

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