Han Shuxin, Gao Yanling, Fan Guoquan, Zhang Wei, Qiu Cailing, Zhang Shu, Bai Yanju, Zhang Junhua, Spetz Carl
College of Agricultural, Northeast Agricultural University, Harbin 150030, China.
Virus-free Seeding Research Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
Plant Pathol J. 2017 Aug;33(4):382-392. doi: 10.5423/PPJ.OA.09.2016.0189. Epub 2017 Aug 1.
This study reports the findings of a distinct (PVY) isolate found in Northeast China. One hundred and ten samples (leaves and tubers) were collected from potato plants showing mosaic symptoms around the city of Harbin in Heilongjiang province of China. The collected tubers were planted and let to grow in a greenhouse. New potato plants generated from these tubers showed similar symptoms, except for one plant. Subsequent serological analyses revealed PVY as the causing agent of the disease. A novel PVY isolate (referred to as HLJ-C-44 in this study) was isolated from this sample showing unique mild mosaic and crisped leaf margin symptoms. The complete genome of this isolate was analyzed and determined. The results showed that HLJ-C-44 is a typical PVY isolate. Phylogenetic analysis indicated that this isolate belongs to the N-Wi strain group of PVY recombinants (PVY) and also shared the highest overall sequence identity (nucleotide and amino acid) with other members of this strain group. However, recombination analysis of isolate HLJ-C-44 revealed a recombination pattern that differed from that of other PVY isolates. Moreover, biological assays in four different potato cultivars and in also revealed a different phenotypic response than that of a typical PVY isolate. This data, combined, suggest that HLJ-C-44 is a novel PVY recombinant with distinct biological properties.
本研究报告了在中国东北地区发现的一种独特的马铃薯Y病毒（PVY）分离株的研究结果。从中国黑龙江省哈尔滨市周边表现出花叶症状的马铃薯植株上采集了110个样本（叶片和块茎）。将采集到的块茎种植在温室中使其生长。由这些块茎产生的新马铃薯植株除一株外均表现出相似症状。随后的血清学分析表明PVY是该病的致病因子。从表现出独特的轻度花叶和叶缘卷曲症状的该样本中分离出一种新型PVY分离株（本研究中称为HLJ-C-44）。对该分离株的完整基因组进行了分析和测定。结果表明HLJ-C-44是一种典型的PVY分离株。系统发育分析表明，该分离株属于PVY重组体（PVY）的N-Wi株系组，并且与该株系组的其他成员也具有最高的总体序列同一性（核苷酸和氨基酸）。然而，对分离株HLJ-C-44的重组分析揭示了一种与其他PVY分离株不同的重组模式。此外，在四个不同马铃薯品种中的生物学测定也显示出与典型PVY分离株不同的表型反应。综合这些数据表明，HLJ-C-44是一种具有独特生物学特性的新型PVY重组体。
