The human lymphocyte micronucleus assay: a comparison between whole-blood and separated-lymphocyte cultures.
作者信息
Migliore L, Nieri M, Amodio S, Loprieno N
机构信息
Dipartimento di Scienze dell'Ambiente e del Territorio, Università di Pisa, Italy.
出版信息
Mutat Res. 1989 Nov;227(3):167-72. doi: 10.1016/0165-7992(89)90041-9.
PMID:2509905
Abstract
The induction of micronuclei (MN) by vincristine, mitomycin C and cyclophosphamide was compared in purified lymphocytes and in whole-blood cultures. With both assays, cytokinesis was blocked by cytochalasin B and MN were only scored in binucleate cells. The data suggest that whole-blood cultures may be considered a better experimental condition for the detection of MN induced by chemicals in vitro.
摘要
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