Dische F E, Brooke I P, Cashman S J, Severn A, Taube D, Parsons V, Kershaw M, Reed A, Pusey C D
Dulwich Hospital, London, UK.
Nephrol Dial Transplant. 1989;4(7):611-7.
A monoclonal antibody, P1, possessing antiglomerular basement membrane specificity similar to that of naturally-occurring Goodpasture antibody, was used to study renal biopsy material from patients with thin-membrane nephropathy. Immunofluorescence and immunoperoxidase techniques were employed to detect tissue localisation after sections had been incubated with P1. Staining of glomerular and tubular basement membranes in the 14 patients with thin-membrane nephropathy was similar to that in 10 subjects with various other renal diseases, whereas three patients with Alport's syndrome all gave diminished or absent staining, as has been reported previously. These observations confirm that Goodpasture antigen is present in the basement membranes in thin-membrane nephropathy and that it reacts normally with P1 antibody. They also add to the evidence that the lesions of thin-membrane nephropathy and Alport's syndrome are fundamentally different. The staining method may be used as a differential diagnostic test.
一种单克隆抗体P1，具有与天然存在的Goodpasture抗体相似的抗肾小球基底膜特异性，被用于研究薄基底膜肾病患者的肾活检材料。在用P1孵育切片后，采用免疫荧光和免疫过氧化物酶技术检测组织定位。14例薄基底膜肾病患者的肾小球和肾小管基底膜染色与10例患有其他各种肾脏疾病的受试者相似，而3例Alport综合征患者的染色均减弱或缺失，正如先前报道的那样。这些观察结果证实，Goodpasture抗原存在于薄基底膜肾病的基底膜中，并且它与P1抗体正常反应。它们还增加了证据，表明薄基底膜肾病和Alport综合征的病变根本不同。这种染色方法可作为一种鉴别诊断试验。
