Hubrecht Institute-KNAW & UMC Utrecht, Utrecht 3584CT, The Netherlands WWU Münster, Institute for Cardiovascular Organogenesis and Regeneration, Faculty of Medicine, Münster 48149, Germany
Max Planck Institute for Heart and Lung Research, Department of Developmental Genetics, Bad Nauheim 61231, Germany.
Development. 2014 Aug;141(16):3103-4. doi: 10.1242/dev.112003.
Morpholino oligomers have been used widely and for many years in the zebrafish community to transiently knock down the function of target genes. It has often been difficult, however, to reliably discriminate between specific and non-specific effects, and thus generally accepted guidelines to control for morpholino side effects do not exist. In light of recent methodologies to generate mutant lines in virtually any zebrafish gene, we discuss these different approaches with a specific focus on how the first description of a loss-of-function phenotype in zebrafish should be accomplished.
吗啉代寡核苷酸在斑马鱼研究领域被广泛应用多年，用于瞬时敲低靶基因的功能。然而，通常很难可靠地区分特异性和非特异性作用，因此，不存在普遍接受的控制吗啉代副作用的指导方针。鉴于最近在几乎任何斑马鱼基因中生成突变系的方法，我们将讨论这些不同的方法，并特别关注如何完成斑马鱼中第一个功能丧失表型的描述。
