Walia A S, Lamon E W
Alcoholism Research Program, VA Medical Center, Birmingham, Alabama.
Proc Soc Exp Biol Med. 1989 Nov;192(2):177-81. doi: 10.3181/00379727-192-42975.
Ethanol inhibits antibody-dependent cell-mediated cytotoxicity in a dose-dependent manner. The inhibitory effect of ethanol was reversed by the addition of excess calcium or calcium ionophore A23187. Excess calcium at 4-8 mM concentrations was required to reverse 50% of the inhibition caused by ethanol. In seven of nine experiments, 16 mM excess calcium completely reversed the inhibition and produced greater lysis than the control. Excess calcium in the absence of ethanol induced a dose-dependent increase in lytic activity by the spleen cells. However, the reversal of inhibition by ethanol could not be attributed to a simple additive effect resulting from the increased cytolytic capacity of the lymphocytes in the presence of excess Ca2+. Ionophore A23187 at 1 microM also partially reversed the inhibitory effect caused by ethanol. Ionophore alone did not potentiate lytic activity. When target cells were not sensitized with antibody, excess calcium had no effect on the lysis of target cells in the presence of ethanol-treated or untreated lymphocytes. These data suggest that ethanol inhibits antibody-dependent cell-mediated cytotoxicity at a calcium-dependent step.
乙醇以剂量依赖的方式抑制抗体依赖性细胞介导的细胞毒性。通过添加过量的钙或钙离子载体A23187可逆转乙醇的抑制作用。需要4 - 8 mM浓度的过量钙来逆转由乙醇引起的50%的抑制作用。在九个实验中的七个实验中，16 mM的过量钙完全逆转了抑制作用，并产生了比对照更大的细胞裂解。在无乙醇的情况下，过量的钙可诱导脾细胞的溶细胞活性呈剂量依赖性增加。然而，乙醇抑制作用的逆转不能归因于在存在过量Ca2+的情况下淋巴细胞溶细胞能力增加所导致的简单相加效应。1 microM的离子载体A23187也部分逆转了由乙醇引起的抑制作用。单独的离子载体不会增强溶细胞活性。当靶细胞未用抗体致敏时，在存在经乙醇处理或未处理的淋巴细胞的情况下，过量的钙对靶细胞的裂解没有影响。这些数据表明，乙醇在一个钙依赖步骤中抑制抗体依赖性细胞介导的细胞毒性。
