Ren Zhe, He Chenghua, Fan Yanhong, Si Huimin, Wang Yuwei, Shi Zhiyu, Zhao Xiumei, Zheng Yating, Liu Qingxin, Zhang Haibin
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Wujiang Agricultural Commission, Wujiang 215200, China.
Int J Biol Macromol. 2014 Sep;70:590-5. doi: 10.1016/j.ijbiomac.2014.07.044. Epub 2014 Aug 4.
The goal of the present study was to investigate the immune-enhancing activity of polysaccharides from Cyrtomium macrophyllum (CMP). Two experiments were carried out. In immunosuppression experiment, the immune-enhancing effect of CMP in immunosuppressive mice was performed. The results showed that CMP at high and medium doses was able to overcome the CY-induced immunosuppression, significantly increases the thymus and spleen indices, enhances lymphocyte proliferation activity and macrophage function, improves immunoglobulin and cytokines levels compared with negative control group. In macrophage immunomodulatory experiment, the immune-enhancing effect of CMP in RAW264.7 cells was measured. The results showed that CMP induced the elevation of NO production, TNF-α secretion and iNOS protein of RAW264.7 cells. CMP can also strongly increase NF-κB levels in nuclear, which is an important transcription factor that can modulate expressions of iNOS, NO and TNF-α. Therefore, the CMP could be an effective immunomodulatory agent.
本研究的目的是探讨大叶贯众多糖（CMP）的免疫增强活性。进行了两个实验。在免疫抑制实验中，研究了CMP对免疫抑制小鼠的免疫增强作用。结果表明，与阴性对照组相比，高剂量和中剂量的CMP能够克服环磷酰胺诱导的免疫抑制，显著增加胸腺和脾脏指数，增强淋巴细胞增殖活性和巨噬细胞功能，提高免疫球蛋白和细胞因子水平。在巨噬细胞免疫调节实验中，检测了CMP对RAW264.7细胞的免疫增强作用。结果表明，CMP诱导RAW264.7细胞一氧化氮（NO）生成增加、肿瘤坏死因子-α（TNF-α）分泌增加和诱导型一氧化氮合酶（iNOS）蛋白表达增加。CMP还能强烈增加细胞核中核因子-κB（NF-κB）水平，NF-κB是一种重要的转录因子，可调节iNOS、NO和TNF-α的表达。因此，CMP可能是一种有效的免疫调节剂。
