Bao Mingming, Zhang Xiujie, Xie Congxin, Wan Shiming, Cai Lingang, Zhou Qiong
a College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , P.R. China .
b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan , P.R. China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1385-6. doi: 10.3109/19401736.2014.947592. Epub 2014 Aug 8.
The complete mitochondrial genome of Schizothorax pseudaksaiensis was cloned and sequenced in the present study. The genome was 16,582 bp in size, which had a mostly conserved structural organization in comparison with that of other teleost fish. It consisted of 37 genes (13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes), and 2 main non-coding regions (the control region and the origin of the light strand replication). All protein-coding genes started with ATG except for COX1, which began with GTG. However, the termination codons of 13 protein-coding genes varied with TAA, TA, T or TAG. The overall base composition of S. pseudaksaiensis in descending order was A 30.18%, C 27.08%, T 25.37% and G 17.37%, with a slight A + T bias. The complete mitochondrial genome sequence may provide useful information for phylogenetic analysis and studies of population genetics of S. pseudaksaiensis.
在本研究中，对似尖裸鲤的完整线粒体基因组进行了克隆和测序。该基因组大小为16,582 bp，与其他硬骨鱼相比，其结构组织大多保守。它由37个基因组成（13个蛋白质编码基因、22个转运RNA基因和2个核糖体RNA基因），以及2个主要的非编码区（控制区和轻链复制起点）。除COX1以GTG起始外，所有蛋白质编码基因均以ATG起始。然而，13个蛋白质编码基因的终止密码子有所不同，分别为TAA、TA、T或TAG。似尖裸鲤的总体碱基组成从高到低依次为：A 30.18%、C 27.08%、T 25.37%和G 17.37%，具有轻微的A+T偏向性。完整的线粒体基因组序列可能为似尖裸鲤的系统发育分析和群体遗传学研究提供有用信息。
