Wang Beibei, Wang Qi, Ma Meihu, Cai Zhaoxia
National R&D Center for Egg Processing, Food Science and Technology College, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, China.
National R&D Center for Egg Processing, Food Science and Technology College, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Jan 25;135:428-34. doi: 10.1016/j.saa.2014.07.024. Epub 2014 Jul 24.
In this paper, we constructed the fluorescence resonance energy transfer (FRET) system between two multi-color quantum dots (QDs) of two sizes for rapid and sensitive detection of Staphylococcus aureus. In this system, green-emitting QDs conjugated with rabbit anti-S. aureus antibodies were used as energy donors while orange-emitting QDs conjugated with goat-anti-rabbit IgG were used as energy acceptors to form FRET system. Pre-binding of Staphylococcus aureus (S. aureus) on the donor occupied the binding sites and thus blocked resonance energy transfer between two colors QDs, leading to the quenching fluorescence of the acceptor. The fluorescence of acceptor has a linear calibration graph with the concentrations of S. aureus from 52 to 2.6×10(5) CFU mL(-1). The low detection limit was 10 CFU/mL. It was worth mentioning that the detection method of S. aureus had been applied to the analysis of apple juice and milk samples, which could potentially be developed into a sensor in the further study.
在本文中,我们构建了两种尺寸的两个多色量子点(QD)之间的荧光共振能量转移(FRET)系统,用于快速、灵敏地检测金黄色葡萄球菌。在该系统中,与兔抗金黄色葡萄球菌抗体偶联的绿色发射量子点用作能量供体,而与山羊抗兔IgG偶联的橙色发射量子点用作能量受体,以形成FRET系统。金黄色葡萄球菌在供体上的预结合占据了结合位点,从而阻断了两种颜色量子点之间的共振能量转移,导致受体荧光猝灭。受体的荧光与金黄色葡萄球菌浓度在52至2.6×10⁵ CFU mL⁻¹之间具有线性校准曲线。最低检测限为10 CFU/mL。值得一提的是,金黄色葡萄球菌的检测方法已应用于苹果汁和牛奶样品的分析,在进一步研究中有可能开发成一种传感器。