Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Food Chem. 2013 Dec 15;141(4):4060-5. doi: 10.1016/j.foodchem.2013.06.135. Epub 2013 Jul 6.
In this paper, we constructed the fluorescence resonance energy transfer (FRET) system between MPA-capped CdTe quantum dots (QDs) and Rhodamine B (RB) in the environment of cetyltrimethylammonium bromide (CTAB). In this system, CdTe QDs were treated as energy donors, whilst RB was treated as energy acceptor. CTAB was used to make the distance between the particles of CdTe QDs and RB closer to improve the efficiency of FRET system. We optimized some important factors which would affect the efficiency of FRET system. Under the optimized experimental conditions, melamine (MB) could be detected based on the fluorescence intensity changes of RB in the FRET system. We could get a linear relationship between the fluorescence intensity of RB and the concentration of MB in the range of 0.05-4.0 μmol/L and the detection limit was 0.01 μmol/L. The proposed method was applied for the determination of melamine in milk samples with satisfactory results.
在本论文中，我们在十六烷基三甲基溴化铵（CTAB）环境中构建了巯基丙酸（MPA）封端的碲化镉量子点（QDs）与若丹明 B（RB）之间的荧光共振能量转移（FRET）体系。在该体系中，CdTe QDs 被视为能量供体，而 RB 则被视为能量受体。CTAB 用于使 CdTe QDs 和 RB 颗粒之间的距离更近，以提高 FRET 体系的效率。我们优化了一些会影响 FRET 体系效率的重要因素。在优化的实验条件下，可基于 FRET 体系中 RB 的荧光强度变化来检测三聚氰胺（MB）。我们可以在 0.05-4.0 μmol/L 的范围内获得 RB 荧光强度与 MB 浓度之间的线性关系，检测限为 0.01 μmol/L。该方法已应用于牛奶样品中三聚氰胺的测定，结果令人满意。
