通过萘-2,3-二甲醛进行色谱前衍生化法测定多巴胺、去甲肾上腺素及相关痕量胺类

Determination of dopamine, norepinephrine, and related trace amines by prechromatographic derivatization with naphthalene-2,3-dicarboxaldehyde.

作者信息

Kawasaki T, Higuchi T, Imai K, Wong O S

机构信息

Center for Bioanalytical Research, University of Kansas, Lawrence 66045.

出版信息

Anal Biochem. 1989 Aug 1;180(2):279-85. doi: 10.1016/0003-2697(89)90431-4.

DOI:10.1016/0003-2697(89)90431-4

PMID:2510545

Abstract

Dopamine, norepinephrine, octopamine, tyramine, and 3,4-dihydroxybenzylamine react readily with naphthalene-2,3-dicarboxaldehyde in the presence of cyanide ion under mild conditions to give highly fluorescent cyanobenz[f]isoindole (CBI) products. The CBI products exhibit good solution chemical stability. The high-fluorescence quantum efficiency of the CBI fluorophore and the ability to excite these adducts in the visible region (420-450 nm) enhance the sensitivity and selectivity of this derivatization detection technique. The CBI products of catecholamines and "trace" amines are readily separated by reverse-phase HPLC giving detection limits in the 20 to 60 fmol range (S/N = 3). A prechromatographic derivatization HPLC assay for the trace analysis of dopamine and norepinephrine in urine is described.

摘要

在温和条件下，多巴胺、去甲肾上腺素、章鱼胺、酪胺和3,4 - 二羟基苄胺在氰离子存在下能与萘-2,3 - 二甲醛迅速反应，生成高荧光的氰苯并[f]异吲哚（CBI）产物。CBI产物具有良好的溶液化学稳定性。CBI荧光团的高荧光量子效率以及在可见光区域（420 - 450 nm）激发这些加合物的能力提高了这种衍生化检测技术的灵敏度和选择性。儿茶酚胺和“痕量”胺的CBI产物很容易通过反相高效液相色谱分离，检测限在20至60飞摩尔范围内（信噪比=3）。本文描述了一种用于尿液中多巴胺和去甲肾上腺素痕量分析的色谱前衍生化高效液相色谱测定法。