Lakhina Vanisha, Subramanian Lakshmi, Huilgol Dhananjay, Shetty Ashwin S, Vaidya Vidita A, Tole Shubha
Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India ; Current affiliation: Lewis Sigler Institute for Integrative Genomics, Princeton University, NJ, USA.
Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India ; Current affiliation: Department of Neurology, University of California, San Francisco, CA, USA.
F1000Res. 2013 Oct 4;2:205. doi: 10.12688/f1000research.2-205.v1. eCollection 2013.
The LIM-homeodomain (LIM-HD) family of transcription factors is well known for its functions during several developmental processes including cell fate specification, cell migration and axon guidance, and its members play fundamental roles in hippocampal development. The hippocampus is a structure that displays striking activity dependent plasticity. We examined whether LIM-HD genes and their co-factors are regulated during kainic acid induced seizure in the adult rat hippocampus as well as in early postnatal rats, when the hippocampal circuitry is not fully developed. We report a distinct and field-specific regulation of LIM-HD genes Lhx1, Lhx2, and Lhx9, LIM-only gene Lmo4, and cofactor Clim1a in the adult hippocampus after seizure induction. In contrast none of these genes displayed altered levels upon induction of seizure in postnatal animals. Our results provide evidence of temporal and spatial seizure mediated regulation of LIM-HD family members and suggest that LIM-HD gene function may be involved in activity dependent plasticity in the adult hippocampus.
转录因子的LIM同源结构域（LIM-HD）家族因其在包括细胞命运决定、细胞迁移和轴突导向在内的多个发育过程中的功能而闻名，其成员在海马体发育中发挥着重要作用。海马体是一个表现出显著的活动依赖性可塑性的结构。我们研究了在成年大鼠海马体以及出生后早期大鼠（此时海马体回路尚未完全发育）中，在 kainic 酸诱导癫痫发作期间，LIM-HD 基因及其辅助因子是否受到调控。我们报告了癫痫发作诱导后成年海马体中 LIM-HD 基因 Lhx1、Lhx2 和 Lhx9、仅含 LIM 的基因 Lmo4 以及辅助因子 Clim1a 的独特且区域特异性调控。相比之下，在出生后动物中诱导癫痫发作时，这些基因均未显示出水平变化。我们的结果提供了癫痫发作介导LIM-HD家族成员的时空调控的证据，并表明LIM-HD基因功能可能参与成年海马体的活动依赖性可塑性。
