心脏停搏液保护下缺血期间心肌磷酸盐代谢的31P-核磁共振波谱分析。
31P-NMR spectroscopy of phosphate compartmentation during ischaemia in hearts protected by cardioplegic treatment.
作者信息
Stier A, Finch S A, Schäfer H, Gebhardt M M, Bretschneider H J
机构信息
Max-Planck-Institut für Biophysikalische Chemie, Göttingen, W. Germany.
出版信息
Free Radic Res Commun. 1989;7(3-6):293-300. doi: 10.3109/10715768909087954.
PMID:2511087
Abstract
Four tissue compartments, differing in proton and inorganic phosphate concentration, were resolved by 31P-NMR spectroscopy in samples from dog hearts after cardioplegic treatment with HTK solution. Inversion of the physiological cytoplasmic-mitochondrial pH gradient was observed. The considerable ensuing acidosis of the matrix is discussed with regard to a possible delocalization of ferrous ions.
摘要
在用HTK溶液进行心脏停搏治疗后，通过31P-NMR光谱法在犬心脏样本中分辨出了四个质子和无机磷酸盐浓度不同的组织区室。观察到生理细胞质-线粒体pH梯度发生了反转。针对亚铁离子可能的离域化，讨论了随之而来的基质显著酸中毒现象。
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