Kawano T, Tajima C, Fukuma K, Yamamoto S, Okamura H
Department of Obstetrics and Gynecology, Kumamoto University Medical School.
Nihon Sanka Fujinka Gakkai Zasshi. 1989 Oct;41(10):1507-11.
To elucidate the effect of the antiprogesterone steroid RU 486 (RU) on endometrial glycogen metabolism, a dose of 30 mg/kg of the agent was administered to pregnant rats on Day 2 (group 1) or Day 4 (group 2) of pregnancy, and glycogen content, glycogen synthetase (GS) and glycogen phosphorylase (GP) activities in the endometrium were investigated on Day 6. In addition, serum ovarian steroid hormones and the number of implantation sites were evaluated. The glycogen content in the endometrium in group 1 and group 2 decreased significantly (p less than 0.02 and p less than 0.05) compared with the control group. The total GS activity in the endometrium in group 1 decreased significantly (p less than 0.05). On the other hand, the independent GS activity in group 2 increased significantly (p less than 0.05) compared with the control group. The active GP activity in group 1 increased markedly, and the total GP activity increased significantly (p less than 0.05) compared with the control value. In group 2, the active and total GP activities increased significantly (p less than 0.01 and p less than 0.02) compared with the control values. Serum progesterone (P) concentrations in group 1 and group 2 were significantly (p less than 0.05 and p less than 0.05) lower than those in the control groups 2 days after the RU administration, and implantations were significantly (p less than 0.01 and p less than 0.01) inhibited. These results suggest that RU 486 affects endometrial glycogen metabolism, resulting in the prevention of implantation.
为阐明抗孕激素甾体RU 486（RU）对子宫内膜糖原代谢的影响，于妊娠第2天（第1组）或第4天（第2组）给妊娠大鼠注射30mg/kg该药物，并于第6天研究子宫内膜中的糖原含量、糖原合成酶（GS）和糖原磷酸化酶（GP）活性。此外，还评估了血清卵巢甾体激素和着床部位数量。与对照组相比，第1组和第2组子宫内膜中的糖原含量显著降低（p<0.02和p<0.05）。第1组子宫内膜中的总GS活性显著降低（p<0.05）。另一方面，与对照组相比，第2组中的独立GS活性显著增加（p<0.05）。第1组中的活性GP活性显著增加，与对照值相比，总GP活性显著增加（p<0.05）。在第2组中，与对照值相比，活性和总GP活性显著增加（p<0.01和p<0.02）。在给予RU后2天，第1组和第2组中的血清孕酮（P）浓度显著低于对照组（p<0.05和p<0.05），着床受到显著抑制（p<0.01和p<0.01）。这些结果表明，RU 486影响子宫内膜糖原代谢，从而阻止着床。
