用于检测具有tetM介导的四环素抗性细菌的快速核酸检测法。
Rapid nucleic acid assay for detection of bacteria with tetM-mediated tetracycline resistance.
作者信息
Sanchez-Pescador R, Running J A, Stempien M M, Urdea M S
机构信息
Chiron Corporation, Emeryville, California 94608.
出版信息
Antimicrob Agents Chemother. 1989 Oct;33(10):1813-5. doi: 10.1128/AAC.33.10.1813.
Abstract
A novel nucleic acid assay has been developed to screen bacterial populations for the presence of the tetM structural gene. The method involves the specific hybridization of several synthetic oligonucleotides to the gene in a crude bacterial lysate solution. As few as 1.5 x 10(4) CFU can be detected with the assay.
摘要
已开发出一种新型核酸检测方法，用于筛选细菌群体中是否存在tetM结构基因。该方法涉及几种合成寡核苷酸与粗制细菌裂解液溶液中的基因进行特异性杂交。该检测方法能够检测低至1.5×10⁴CFU（菌落形成单位）的细菌。
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