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克氏原螯虾转录组分析揭示了性腺发育相关基因。

Transcriptome analysis of red swamp crawfish Procambarus clarkii reveals genes involved in gonadal development.

作者信息

Jiang Hucheng, Xing Zhijun, Lu Wei, Qian Zhaojun, Yu Hongwei, Li Jiale

机构信息

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai, China.

Jiangsu Xuyi Riverred Crawfish Eco-Park CO. LTD, Xuyi, China.

出版信息

PLoS One. 2014 Aug 13;9(8):e105122. doi: 10.1371/journal.pone.0105122. eCollection 2014.

DOI:10.1371/journal.pone.0105122
PMID:25118947
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4132113/
Abstract

BACKGROUND

The red swamp crawfish, Procambarus clarkii, has become one of the most economically important cultured species in China. Currently, little is known about the gonadal development of this species. Isolation and characterization of genes are an initial step towards understanding gonadal development of P. clarkii.

RESULTS

Using the 454 pyrosequencing technology, we obtained a total of 1,134,993 high quality sequence reads from the crawfish testis and ovary libraries. We aimed to identify different genes with a potential role in gonad development. The assembly formed into 22,652 isotigs, distributed by GO analysis across 55 categories in the three ontologies, 'molecular function', 'cellular component', and 'biological processes'. Comparative transcript analysis showed that 1,720 isotigs in the ovary were up-regulated and 2138 isotigs were down-regulated. Several gonad development related genes, such as vitellogenin, cyclin B, cyclin-dependent kinases 2, Dmc1 and ubiquitin were identified. Quantitative real-time PCR verified the expression profiles of 14 differentially expressed genes, and confirmed the reliability of the 454 pyrosequencing.

CONCLUSIONS

Our findings provide an archive for future research on gonadal development at a molecular level in P. clarkii and other crustacean. This data will be helpful to develop new ideas for artificial regulation of the reproductive process in crawfish aquaculture.

摘要

背景

克氏原螯虾已成为中国最重要的经济养殖物种之一。目前，对于该物种的性腺发育了解甚少。基因的分离与特性分析是了解克氏原螯虾性腺发育的第一步。

结果

利用454焦磷酸测序技术，我们从螯虾的精巢和卵巢文库中总共获得了1,134,993条高质量序列读数。我们旨在鉴定在性腺发育中具有潜在作用的不同基因。组装形成了22,652个重叠群，通过GO分析分布在“分子功能”、“细胞成分”和“生物学过程”这三个本体论的55个类别中。比较转录本分析表明，卵巢中有1,720个重叠群上调，2,138个重叠群下调。鉴定出了几个与性腺发育相关的基因，如卵黄蛋白原、细胞周期蛋白B、细胞周期蛋白依赖性激酶2、Dmc1和泛素。实时定量PCR验证了14个差异表达基因的表达谱，并证实了454焦磷酸测序的可靠性。

结论

我们的研究结果为今后在分子水平上研究克氏原螯虾及其他甲壳类动物的性腺发育提供了资料。这些数据将有助于为小龙虾养殖中生殖过程的人工调控开发新思路。

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Genet Mol Res. 2013 Oct 30;12(4):5215-25. doi: 10.4238/2013.October.30.6.
2
Characterization and expression of cell division cycle 2 (Cdc2) mRNA and protein during ovarian development of the giant tiger shrimp Penaeus monodon.在斑节对虾卵巢发育过程中细胞分裂周期 2 (Cdc2) mRNA 和蛋白的鉴定和表达。
Gen Comp Endocrinol. 2013 Nov 1;193:103-11. doi: 10.1016/j.ygcen.2013.07.012. Epub 2013 Jul 27.
3
Genes (Basel). 2023 Dec 8;14(12):2190. doi: 10.3390/genes14122190.
4
Comparison of assembly using long-read shotgun metagenomic sequencing of viruses in fecal and serum samples from marine mammals.利用长读长鸟枪法宏基因组测序对海洋哺乳动物粪便和血清样本中的病毒进行组装的比较。
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5
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4
The cloning of the cdk2 transcript and the localization of its expression during gametogenesis in the freshwater giant prawn, Macrobrachium rosenbergii.在淡水巨型虾 Macrobrachium rosenbergii 的配子发生过程中克隆 cdk2 转录本及其表达的定位。
Mol Biol Rep. 2013 Aug;40(8):4781-90. doi: 10.1007/s11033-013-2574-7. Epub 2013 May 8.
5
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6
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9
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10
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