He Yuan, Wang Kan, Yan Nieng
State Key Laboratory of Bio-membrane and Membrane Biotechnology, Tsinghua university, Beijing, 100084, China.
Protein Cell. 2014 Sep;5(9):658-72. doi: 10.1007/s13238-014-0086-4. Epub 2014 Aug 15.
Eukaryotic membrane proteins, many of which are key players in various biological processes, constitute more than half of the drug targets and represent important candidates for structural studies. In contrast to their physiological significance, only very limited number of eukaryotic membrane protein structures have been obtained due to the technical challenges in the generation of recombinant proteins. In this review, we examine the major recombinant expression systems for eukaryotic membrane proteins and compare their relative advantages and disadvantages. We also attempted to summarize the recent technical strategies in the advancement of eukaryotic membrane protein purification and crystallization.
真核膜蛋白是各种生物过程中的关键参与者，构成了一半以上的药物靶点，是结构研究的重要候选对象。与它们的生理意义形成对比的是，由于重组蛋白生成过程中的技术挑战，仅获得了非常有限数量的真核膜蛋白结构。在本综述中，我们研究了真核膜蛋白的主要重组表达系统，并比较了它们的相对优缺点。我们还试图总结真核膜蛋白纯化和结晶进展中的最新技术策略。
