冷冻电镜进入新时代。

Cryo-EM enters a new era.

作者信息

Kühlbrandt Werner

机构信息

Werner Kuehlbrandt is in the Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt, Germany

出版信息

Elife. 2014 Aug 13;3:e03678. doi: 10.7554/eLife.03678.

DOI:10.7554/eLife.03678

PMID:25122623

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4131193/

Abstract

Advances in detector hardware and image-processing software have led to a revolution in the use of electron cryo-microscopy to determine complex molecular structures at high resolution.

摘要

探测器硬件和图像处理软件的进步，引发了一场利用冷冻电子显微镜在高分辨率下测定复杂分子结构的革命。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9862/4131193/8cd5277377eb/elife03678f001.jpg

相似文献

Cryo-EM enters a new era.

Elife. 2014 Aug 13;3:e03678. doi: 10.7554/eLife.03678.

Beam-induced motion correction for sub-megadalton cryo-EM particles.

Elife. 2014 Aug 13;3:e03665. doi: 10.7554/eLife.03665.

Processing of Structurally Heterogeneous Cryo-EM Data in RELION.

Methods Enzymol. 2016;579:125-57. doi: 10.1016/bs.mie.2016.04.012. Epub 2016 May 31.

A Super-Clustering Approach for Fully Automated Single Particle Picking in Cryo-EM.

Genes (Basel). 2019 Aug 30;10(9):666. doi: 10.3390/genes10090666.

Single-particle reconstruction statistics: a diagnostic tool in solving biomolecular structures by cryo-EM.

Acta Crystallogr F Struct Biol Commun. 2019 Jan 1;75(Pt 1):33-44. doi: 10.1107/S2053230X18017636.

Mammalian Respiratory Complex I Through the Lens of Cryo-EM.

Annu Rev Biophys. 2019 May 6;48:165-184. doi: 10.1146/annurev-biophys-052118-115704. Epub 2019 Feb 20.

DeepPicker: A deep learning approach for fully automated particle picking in cryo-EM.

J Struct Biol. 2016 Sep;195(3):325-336. doi: 10.1016/j.jsb.2016.07.006. Epub 2016 Jul 14.

A particle-filter framework for robust cryo-EM 3D reconstruction.

Nat Methods. 2018 Dec;15(12):1083-1089. doi: 10.1038/s41592-018-0223-8. Epub 2018 Nov 30.

Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud.

Elife. 2015 May 8;4:e06664. doi: 10.7554/eLife.06664.

Semi-automated selection of cryo-EM particles in RELION-1.3.

J Struct Biol. 2015 Feb;189(2):114-22. doi: 10.1016/j.jsb.2014.11.010. Epub 2014 Dec 6.

引用本文的文献

Present and future structural biology activities at DESY and the European XFEL.

J Synchrotron Radiat. 2025 Mar 1;32(Pt 2):474-485. doi: 10.1107/S1600577525000669. Epub 2025 Feb 18.

Protein Secondary Structure and DNA/RNA Detection for Cryo-EM and Cryo-ET Using Emap2sec and Emap2sec.

Methods Mol Biol. 2025;2867:105-120. doi: 10.1007/978-1-0716-4196-5_6.

EMhub: a web platform for data management and on-the-fly processing in scientific facilities.

Acta Crystallogr D Struct Biol. 2024 Nov 1;80(Pt 11):780-790. doi: 10.1107/S2059798324009471. Epub 2024 Oct 7.

Photosystem I: A Paradigm for Understanding Biological Environmental Adaptation Mechanisms in Cyanobacteria and Algae.

Int J Mol Sci. 2024 Aug 12;25(16):8767. doi: 10.3390/ijms25168767.

Cryo-electron microscopy in the study of virus entry and infection.

Front Mol Biosci. 2024 Jul 24;11:1429180. doi: 10.3389/fmolb.2024.1429180. eCollection 2024.

The myth of high-resolution liquid phase biological electron microscopy.

Protein Sci. 2024 Aug;33(8):e5125. doi: 10.1002/pro.5125.

Cryo-EM structures of pathogenic fibrils and their impact on neurodegenerative disease research.

Neuron. 2024 Jul 17;112(14):2269-2288. doi: 10.1016/j.neuron.2024.05.012. Epub 2024 Jun 3.

Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines.

Adv Exp Med Biol. 2024;3234:173-190. doi: 10.1007/978-3-031-52193-5_12.

Cryo-electron microscopy-based drug design.

Front Mol Biosci. 2024 Mar 4;11:1342179. doi: 10.3389/fmolb.2024.1342179. eCollection 2024.

Quantitative analysis of protein dynamics using a deep learning technique combined with experimental cryo-EM density data and MD simulations.

Biophys Physicobiol. 2023 May 16;20(2):e200022. doi: 10.2142/biophysico.bppb-v20.0022. eCollection 2023.

本文引用的文献

Beam-induced motion correction for sub-megadalton cryo-EM particles.

Elife. 2014 Aug 13;3:e03665. doi: 10.7554/eLife.03665.

Three-dimensional structure of human γ-secretase.

Nature. 2014 Aug 14;512(7513):166-170. doi: 10.1038/nature13567. Epub 2014 Jun 29.

Structure of the mammalian ribosome-Sec61 complex to 3.4 Å resolution.

Cell. 2014 Jun 19;157(7):1632-43. doi: 10.1016/j.cell.2014.05.024. Epub 2014 Jun 12.

Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine.

Elife. 2014 Jun 9;3:e03080. doi: 10.7554/eLife.03080.

Structure of the yeast mitochondrial large ribosomal subunit.

Science. 2014 Mar 28;343(6178):1485-1489. doi: 10.1126/science.1249410.

Biochemistry. The resolution revolution.

Science. 2014 Mar 28;343(6178):1443-4. doi: 10.1126/science.1251652.

Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector.

Elife. 2014 Feb 25;3:e01963. doi: 10.7554/eLife.01963.

TRPV1 structures in distinct conformations reveal activation mechanisms.

Nature. 2013 Dec 5;504(7478):113-8. doi: 10.1038/nature12823.

Structure of the TRPV1 ion channel determined by electron cryo-microscopy.

Nature. 2013 Dec 5;504(7478):107-12. doi: 10.1038/nature12822.

Molecular architecture of a eukaryotic translational initiation complex.

Science. 2013 Nov 15;342(6160):1240585. doi: 10.1126/science.1240585. Epub 2013 Nov 7.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验

冷冻电镜进入新时代。

Cryo-EM enters a new era.

作者信息

机构信息

出版信息

相似文献

引用本文的文献

本文引用的文献

文献AI研究员

用中文搜PubMed

文档翻译

Suppr 超能文献

冷冻电镜进入新时代。

Cryo-EM enters a new era.

作者信息

机构信息

出版信息

相似文献

引用本文的文献

本文引用的文献