Smirnov V B
Zh Evol Biokhim Fiziol. 1989 Jul-Aug;25(4):549-51.
Isolated thoracic ganglia were incubated in physiological solution containing 14C-tryptophan. After this procedure, they were homogenized in a 1 per cent solution of HCL in methanol, and supernatant was subjected to two-dimensional thin layer chromatography in the presence of tryptophan, kynurenine, 3-hydroxy kynurenine, as well as kynurenic, anthranilic and xanthurenic acids. The spots were cut out and counted by liquid scintillation technique. Except tryptophan, only kynurenine and 3-hydroxy kynurenine spots contained notable radioactivity. Therefore, at least the initial stages of kynurenine pathway operate in the nervous system of Drosophila melanogaster. This finding is in accordance with observations of the effects of kynurenines on insect behaviour.
将离体的胸神经节置于含有14C-色氨酸的生理溶液中孵育。此步骤完成后，将它们在1%的盐酸甲醇溶液中匀浆，然后将上清液在色氨酸、犬尿氨酸、3-羟基犬尿氨酸以及犬尿酸、邻氨基苯甲酸和黄尿酸存在的情况下进行二维薄层色谱分析。将斑点切下并通过液体闪烁技术进行计数。除色氨酸外，只有犬尿氨酸和3-羟基犬尿氨酸斑点含有显著的放射性。因此，至少犬尿氨酸途径的初始阶段在黑腹果蝇的神经系统中起作用。这一发现与关于犬尿氨酸对昆虫行为影响的观察结果一致。
