Levesque M J, Groom A C
Can J Physiol Pharmacol. 1978 Apr;56(2):260-8. doi: 10.1139/y78-038.
On perfusion of isolated, denervated spleens with Ringer solution, immature and abnormal red cells are released into the venous outflow much more slowly than normal mature cells, being delayed through adherence to fine structures of the red pulp (Am. J. Physiol. 231, 1665-1671 (1976)). Evidence suggested that the rate at which such cells are released from the 'bound' state might depend on local pH and fluid shear rate within the pulp. Therefore, the rate of washout for this slow component of red cells, from cat spleens, was measured as a function of pH and flow rate of the perfusate. The volume of solution (V 1/2) for 50% washout of 'bound' cells decreased as pH was lowered from 7.8 to 6.6, especially (from 97 to 18 ml/g) between 7.4 and 6.6. The percentage total red cell outflow thus represented rose from 0.06 to 0.5 as pH fell from 7.8 to 6.6. At a high perfusion rate (14-16 ml/min) the V 1/2 value was only one-half that prevailing at a lower rate (4-6 ml/min), and the percentage flow of 'bound' red cells was more than three times greater. Both acidic pH and augmented blood flow thus assist release of adherent red cells from the splenic pulp.
用林格溶液灌注分离的、去神经的脾脏时，未成熟和异常红细胞释放到静脉流出液中的速度比正常成熟细胞慢得多，这是因为它们黏附于脾髓的精细结构而被延迟（《美国生理学杂志》231, 1665 - 1671 (1976)）。有证据表明，这类细胞从“结合”状态释放的速率可能取决于脾髓内的局部pH值和流体剪切速率。因此，测定了猫脾脏中红细胞这种缓慢成分的洗脱速率与灌注液pH值和流速的函数关系。随着pH值从7.8降至6.6，“结合”细胞50%洗脱所需的溶液体积（V 1/2）减小，尤其是在7.4至6.6之间（从97降至18 ml/g）。随着pH值从7.8降至6.6，红细胞流出总量的百分比从0.06升至0.5。在高灌注速率（14 - 16 ml/min）下，V 1/2值仅为低灌注速率（4 - 6 ml/min）时的一半，“结合”红细胞的流量百分比则增加了三倍多。因此，酸性pH值和增加的血流量都有助于脾髓中黏附红细胞的释放。
