Janczak Dariusz, Ziolkowski Piotr, Garcarek Jerzy, Janczak Dawid, Dorobisz Karolina, Chabowski Mariusz
Department of Surgery, 4th Military Academic Hospital, 5 Weigla street, 50-981 Wroclaw, Poland.
J Cardiothorac Surg. 2014 Aug 15;9:139. doi: 10.1186/1749-8090-9-139.
The aim of the study was the evaluation of the inflammatory cytokines within atheromatic carotid plaque.
The experiment was carried out on 100 symptomatic patients with internal carotid artery stenosis that underwent carotid endarterectomy. Every patient had the wall of the carotid artery resected during organ harvesting surgery in order to evaluate some cytokines (TGF-β, VEGF, FGF, TNF-α) and to perform the immunohistochemistry (IHC). An immunoreactive score (IRS) was calculated based on the staining intensity and the number of cells stained. Over a 3-year period, 7 patients died, and 2 patients were lost to follow-up. The study group consisted of 91 patients. The control group comprised 20 young organ donors with confirmed death brain, who had their normal carotid artery sampled.
In all healthy donors (control group) with normal carotid arteries the three cytokines (TGF-β, VEGF, TNF-α) were not discovered. The presence of FGF was confirmed in 25% of healthy donors, probably due to an intima fibroblasts activity, responsible for the synthesis of elastin and collagen to the extracellular matrix (ECM). Only three cytokines (TGF-β, FGF, TNF-α) were found within atheromatous plaques (study group).
Our research confirmed that these factors may accelerate the development of atheromatic plaque and its destabilisation.
本研究的目的是评估动脉粥样硬化颈动脉斑块内的炎性细胞因子。
对100例有症状的颈内动脉狭窄患者进行了实验，这些患者均接受了颈动脉内膜切除术。在器官获取手术过程中，每位患者都切除了颈动脉壁，以评估某些细胞因子（转化生长因子-β、血管内皮生长因子、成纤维细胞生长因子、肿瘤坏死因子-α）并进行免疫组织化学（IHC）检测。根据染色强度和染色细胞数量计算免疫反应评分（IRS）。在3年期间，7例患者死亡，2例患者失访。研究组由91例患者组成。对照组包括20名确诊脑死亡的年轻器官捐献者，他们的正常颈动脉被取样。
在所有颈动脉正常的健康捐献者（对照组）中，未发现三种细胞因子（转化生长因子-β、血管内皮生长因子、肿瘤坏死因子-α）。25%的健康捐献者中证实存在成纤维细胞生长因子，这可能是由于内膜成纤维细胞的活性，其负责向细胞外基质（ECM）合成弹性蛋白和胶原蛋白。在动脉粥样硬化斑块内（研究组）仅发现三种细胞因子（转化生长因子-β、成纤维细胞生长因子、肿瘤坏死因子-α）。
我们的研究证实，这些因素可能加速动脉粥样硬化斑块的发展及其不稳定。
