巴黎七叶皂苷 VII 抑制人宫颈癌 Hela 细胞的生长。
Paris saponin VII suppressed the growth of human cervical cancer Hela cells.
机构信息
Department of Pathogen Biology and Immunology, Xi'an Medical University, Xi'an, Shaanxi 710021, People's Republic of China.
出版信息
Eur J Med Res. 2014 Aug 15;19(1):41. doi: 10.1186/2047-783X-19-41.
BACKGROUND
Saponins of several herbs are known to induce apoptosis in many cancer cells. The present study aimed to investigate the growth inhibitory effect of Paris saponin VII (PS VII), a kind of steroidal saponins from Chonglou (Rhizoma Paridis Chonglou), on the human cervical cancer cell line Hela and the relative molecular mechanisms.
METHODS
Hela cells were exposed to different concentrations of PS VII (1 to 100 μM). Inhibition of cell proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-ethynyl-2'-deoxyuridine (EdU) assays. The amount of apoptotic cells was evaluated by flow cytometric analysis. And the protein level of cleaved caspase-3, cleaved caspase-9, Bax, and Bcl-2 was evaluated by Western blot.
RESULTS
The half maximal inhibitory concentration (IC50) value of PS VII for the growth inhibition of Hela cells was 2.62 ± 0.11 μM. PS VII increased the expression of caspase-3, caspase-9, and Bax while decreased that of Bcl-2, suggesting that PS VII may induce apoptosis through intrinsic apoptotic ways.
CONCLUSIONS
These data indicate that PS VII has the potential for the treatment of cervical cancer.
背景
几种草药的皂角苷已被证实能诱导许多癌细胞凋亡。本研究旨在探讨重楼甾体皂角苷 PS VII（一种来自重楼的甾体皂角苷）对人宫颈癌 Hela 细胞系的生长抑制作用及其相关分子机制。
方法
用不同浓度的 PS VII（1 至 100 μM）处理 Hela 细胞。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）和 5-乙炔基-2'-脱氧尿苷（EdU）检测抑制细胞增殖的情况。通过流式细胞术分析评估细胞凋亡的数量。用 Western blot 检测裂解的 caspase-3、裂解的 caspase-9、Bax 和 Bcl-2 的蛋白水平。
结果
PS VII 抑制 Hela 细胞生长的半最大抑制浓度（IC50）值为 2.62±0.11 μM。PS VII 增加了 caspase-3、caspase-9 和 Bax 的表达，同时降低了 Bcl-2 的表达，表明 PS VII 可能通过内在凋亡途径诱导细胞凋亡。
结论
这些数据表明 PS VII 具有治疗宫颈癌的潜力。
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