Phytochemicals and Food Quality Group, Instituto de la Grasa (CSIC), 41013 Seville, Spain.
Unité de Physiologie et de Biochimie de la Réponse des Plantes aux Contraintes Abiotiques, Faculté des Sciences de Tunis, Université Tunis El Manar, Tunis 2092, Tunisia.
Molecules. 2024 Feb 10;29(4):817. doi: 10.3390/molecules29040817.
In this study, was characterized concerning its phytochemical composition, antioxidant potential, cytotoxicity, and pancreatic lipase inhibitory activities. Twenty-seven compounds were identified and quantified by HPLC-DAD-MS in the leaf, stem, pericarp, and rhizome of ethanolic extracts. Seven steroidal saponins were detected, and the highest content was quantified in rhizome and pericap. also contained significant amounts of flavonoids in the aerial part. Isorhamnetin tetra-glycoside, quercetin-3-glucosyl-rutinoside, and rutin were the main flavonoid derivatives in leaf, stem, and pericarp extracts, respectively. In addition, eleven phenolic acids were also detected; among them, caffeic acid, protocatechuic acid, p-hydroxybenzoic acid, and ferulic acid were the predominant phenolics, with these having the highest amounts quantified in the rhizome extracts. All the tested extracts possessed antioxidant capacities, with pericarp and rhizome extracts exhibiting the highest activity in DPPH, ABTS, and FRAP assays. The extracts from pericarp and rhizome were revealed to also be the strongest inhibitors of pancreatic lipase. The rhizome extracts exhibited potent cytotoxic activity against HCT-116 and HepG2 with IC50 values of 30 and 54 µg/mL after 48 h of treatment. The present study demonstrated that can be used as a new source of natural antioxidants and potential anticancer and antiobesity compounds.
在这项研究中，对其进行了化学成分分析、抗氧化能力、细胞毒性和胰脂肪酶抑制活性的研究。通过 HPLC-DAD-MS 在叶、茎、果皮和根茎的乙醇提取物中鉴定和定量了 27 种化合物。检测到七种甾体皂苷，其含量在根茎和果皮中最高。还在地上部分含有大量的类黄酮。根状茎中异鼠李素四糖苷、槲皮素-3-葡萄糖基芦丁苷和芦丁的含量最高。此外，还检测到 11 种酚酸；其中，咖啡酸、原儿茶酸、对羟基苯甲酸和阿魏酸是主要的酚酸，其在根茎提取物中的含量最高。所有测试的提取物都具有抗氧化能力，果皮和根茎提取物在 DPPH、ABTS 和 FRAP 测定中表现出最高的活性。果皮和根茎提取物对胰脂肪酶的抑制作用最强。根茎提取物对 HCT-116 和 HepG2 的细胞毒性活性最强，在 48 小时处理后，IC50 值分别为 30 和 54 µg/mL。本研究表明，可能被用作天然抗氧化剂和潜在的抗癌和抗肥胖化合物的新来源。
