Li Kui-Peng, Sun Xiao-Mei, Han Hua, Zhang Shou-Gong
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, PR China; Research Institute of Forestry, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, PR China.
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, PR China.
Gene. 2014 Nov 10;551(2):111-8. doi: 10.1016/j.gene.2014.08.023. Epub 2014 Aug 13.
The full-length cDNA and genomic sequences of the BABY BOOM (BBM) gene, designated LkBBM, were isolated from Larix kaempferi × Larix olgensis. The 3324 bp cDNA was cloned and its open reading frame (ORF) consists of 2370 nucleotides. The deduced 789 amino acid protein contains two AP2 domains and a BBM specific motif. Four conserved motifs between BBM and PLT were identified, which may be conducive to the similar function of BBM and PLT. The three dimensional (3D) structure of LkBBM was predicted and β-sheets in the AP2-R2 domain of LkBBM might recognize the specific base pairs in the major groove. Analysis of the LkBBM gene structure indicates that the gene has eight introns and nine exons. In the 5'-flanking promoter region of LkBBM, many important potential cis-acting elements were identified, such as the TATABOX5 element (a functional TATA element), ROOTMOTIFTAPOX1 element (element of root specificity), AUXREPSIAA4 element (element involved in auxin responsiveness and gene expression in root meristem), MYB1AT element (element involved in MYB recognition), ARR1AT element (element involved in cytokinin responsiveness), GARE1OSREP1 element (element involved in gibberellin responsiveness) and PYRIMIDINEBOXHVEPB1 element (element involved in abscisic acid responsiveness), which all suggested that the expression of LkBBM is highly regulated. Compared with gene expression levels in the stem, stem tip and leaf, LkBBM shows a specific expression in the root, which indicates that LkBBM plays a key role in regulating the development and growth of root in larch. In the processing of larch adventitious root formation, LkBBM started to express on the eighth day after rooting treatment and its transcript level increased continuously afterwards. According to the gene characteristics, LkBBM is proposed as a molecular marker for root primordia of larch, and the initial period of LkBBM expression may be the formation period of root primordia in the processing of adventitious rooting of larch.
从日本落叶松×长白落叶松中分离出命名为LkBBM的BABY BOOM（BBM）基因的全长cDNA和基因组序列。克隆了3324 bp的cDNA，其开放阅读框（ORF）由2370个核苷酸组成。推导的789个氨基酸的蛋白质包含两个AP2结构域和一个BBM特异性基序。鉴定出BBM和PLT之间的四个保守基序，这可能有助于BBM和PLT发挥相似功能。预测了LkBBM的三维（3D）结构，LkBBM的AP2-R2结构域中的β-折叠可能识别大沟中的特定碱基对。LkBBM基因结构分析表明该基因有8个内含子和9个外显子。在LkBBM的5'侧翼启动子区域，鉴定出许多重要的潜在顺式作用元件，如TATABOX5元件（功能性TATA元件）、ROOTMOTIFTAPOX1元件（根特异性元件）、AUXREPSIAA4元件（参与生长素反应和根分生组织中基因表达的元件）、MYB1AT元件（参与MYB识别的元件）、ARR1AT元件（参与细胞分裂素反应的元件）、GARE1OSREP1元件（参与赤霉素反应的元件）和PYRIMIDINEBOXHVEPB1元件（参与脱落酸反应的元件），这些都表明LkBBM的表达受到高度调控。与茎、茎尖和叶中的基因表达水平相比，LkBBM在根中特异性表达，这表明LkBBM在调控落叶松根的发育和生长中起关键作用。在落叶松不定根形成过程中，LkBBM在生根处理后第8天开始表达，其转录水平随后持续增加。根据该基因的特性，LkBBM被提议作为落叶松根原基的分子标记，LkBBM表达的初期可能是落叶松不定根形成过程中根原基的形成期。
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