National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
Gene. 2013 Feb 10;514(2):91-8. doi: 10.1016/j.gene.2012.11.008. Epub 2012 Nov 29.
The plant hormone auxin plays a key role in adventitious rooting. To increase our understanding of genes involved in adventitious root formation, we identified transcripts differentially expressed in single nodal cuttings of Camellia sinensis treated with or without indole-3-butyric acid (IBA) by suppressive subtractive hybridization (SSH). A total of 77 differentially expressed transcripts, including 70 up-regulated and 7 down-regulated sequences, were identified in tea cuttings under IBA treatment. Seven candidate transcripts were selected and analyzed for their response to IBA, and IAA by real time RT-PCR. All these transcripts were up regulated by at least two folds one day after IBA treatment. Meanwhile, IAA showed less positive effects on the expression of candidate transcripts. The full-length cDNA of a F-box/kelch gene was also isolated and found to be similar to a group of At1g23390 like genes. These unigenes provided a new source for mining genes related to adventitious root formation, which facilitate our understanding of relative fundamental metabolism.
植物激素生长素在不定根形成中起关键作用。为了增加我们对参与不定根形成的基因的了解，我们通过抑制差减杂交（SSH）鉴定了用或不用吲哚-3-丁酸（IBA）处理的茶树单节插条中差异表达的转录本。在 IBA 处理下，茶树插条中鉴定出了 77 个差异表达的转录本，包括 70 个上调和 7 个下调序列。选择了 7 个候选转录本并通过实时 RT-PCR 分析它们对 IBA 和 IAA 的反应。所有这些转录本在 IBA 处理后一天至少上调了两倍。同时，IAA 对候选转录本的表达的正向影响较小。还分离并鉴定了一个 F-box/kelch 基因的全长 cDNA，发现它与一组 At1g23390 样基因相似。这些基因提供了一个新的来源来挖掘与不定根形成相关的基因，这有助于我们理解相关的基础代谢。
