Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
J Colloid Interface Sci. 2014 Nov 1;433:183-188. doi: 10.1016/j.jcis.2014.07.033. Epub 2014 Jul 31.
DNA-templated silver nanoclusters (AgNC) are a class of subnanometer sized fluorophores with good photostability and brightness. It has been applied as a diagnostic tool mainly for deoxyribonucleic acid (DNA) detection. Integration of DNA oligomers to generate AgNCs is interesting as varying DNA sequences can result in different fluorescence spectra. This allows a simple fluorescence shifting effect to occur upon DNA hybridization with the hybridization efficiency being a pronominal factor for successful shifting. The ability to shift the fluorescence spectra as a result of hybridization overcomes the issue of background intensities in most fluorescent based assays. Here we describe an optimized method for the detection of single-stranded and double-stranded synthetic forkhead box P3 (FOXP3) target by hybridization with the DNA fluorescence shift sensor. The system forms a three-way junction by successful hybridization of AgNC, G-rich strand (G-rich) to the target DNA, which generated a shift in fluorescence spectra with a marked increase in fluorescence intensity. The DNA fluorescence shift sensor presents a rapid and specific alternative to conventional DNA detection.
基于 DNA 的银纳米团簇（AgNC）是一类亚纳米级的荧光团，具有良好的光稳定性和亮度。它已被用作一种诊断工具，主要用于脱氧核糖核酸（DNA）检测。将 DNA 寡聚体整合以生成 AgNC 很有趣，因为不同的 DNA 序列可以产生不同的荧光光谱。这允许在 DNA 杂交时发生简单的荧光移动效应，杂交效率是成功移动的重要因素。由于杂交而移动荧光光谱的能力克服了大多数基于荧光的测定法中背景强度的问题。在这里，我们描述了一种通过与 DNA 荧光位移传感器杂交来检测单链和双链合成叉头框 P3（FOXP3）靶标的优化方法。该系统通过 AgNC、富含 G 链（G-rich）与靶 DNA 的成功杂交形成三链结，从而产生荧光光谱的移动，荧光强度明显增加。DNA 荧光位移传感器提供了一种快速且特异性的替代传统 DNA 检测方法。
