Urology Department, Shanghai Pudong Hospital of traditional Chinese medicine, Shanghai 201299, China.
Urology Department, Shuguang Hospital affiliated to Shanghai Traditional Chinese Medicine University, Shanghai 201203, China.
Asian Pac J Trop Med. 2014 Oct;7(10):792-5. doi: 10.1016/S1995-7645(14)60138-3.
To observe the intervention influence and effect of simvastatin on the expression of interleukin 17 (LI17), high mobility group protein 1 (HMGB1) and TLR4 path in Lupus nephritis (LN) rats.
A total of 28 BSXSB male mice with LN (16 weeks) were randomly divided into observation group and the comparison group, observation group was given 6 mg•kg(-1)•d(-1) simvastatin in 0.1% PBS lavage for 4 weeks, the comparison group was not given any treatment. Blood urea nitrogen (BUN) level and urine trace albumin (Scr) level of two groups were determined. The expression of IL17, HMGB1 and TLR4 protein was detected using immune histochemical method, and the kidney histological damage was observed.
BNU, LI17, HMGB1, TLR4 protein and HMGB1 mRNA in observation group was significantly lower than that in control group (P<0.05); There was no statistical difference of Scr level between two groups (P>0.05). Histological observation showed glomerular lesions integral of observation group was obviously lower than that of control group.
Simvastatin can reduce the expression of IL17, HMGB1 and TLR4 protein in LN mice, thereby can inhibit the autoimmune response as a potential treatment function of LN.
观察辛伐他汀对狼疮肾炎（LN）大鼠白细胞介素 17（IL17）、高迁移率族蛋白 1（HMGB1）和 TLR4 通路表达的干预影响及作用。
将 28 只 BSXSB 雄性 LN 大鼠（16 周龄）随机分为观察组和对照组，观察组给予 6mg•kg(-1)•d(-1)辛伐他汀 0.1%PBS 灌胃 4 周，对照组不给予任何处理。测定两组大鼠血尿素氮（BUN）水平和尿微量白蛋白（Scr）水平，免疫组化法检测 IL17、HMGB1 和 TLR4 蛋白的表达，观察肾脏组织学损伤。
观察组 BUN、LI17、HMGB1、TLR4 蛋白及 HMGB1mRNA 表达均明显低于对照组（P<0.05）；两组 Scr 水平比较差异无统计学意义（P>0.05）。组织学观察显示观察组肾小球病变积分明显低于对照组。
辛伐他汀可降低 LN 小鼠 IL17、HMGB1 和 TLR4 蛋白的表达，从而可能作为 LN 的一种潜在治疗作用抑制自身免疫反应。
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