Wang Qiang, Yao Yong-ming, Wang Wen-jiang, Xian Li-ming, Dong Ning, Xu Shan, Dou Ke-feng
Department of Basic Research, Bumrns Institute, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100037, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2007 Aug;29(4):478-83.
To investigate the change in renal high mobility group box-1 protein (HMGB1) levels, and the effect of Chinese traditional medicine-Xuebijing injection on HMGB1 expression as well as acute kidney injury in rats after scald injury.
Wistar rats were subjected to 30% full-thickness scald injury followed with delayed resuscitation. Totally 78 animals were divided into sham scald group (n=18), scald injury group (n=30), and Xuebijing injection treatment group (n=30). All animals were sacrificed at 8, 24, and 72 hours postburn. Renal tissue and blood samples were harvested to determine HMGB1 mRNA as well as protein expression and organ functional parameters. HMGB1 mRNA level was semi-quantitatively measured by the reverse transcription polymerase chain reaction taking GAPDH as an internal standard, and protein expressions of HMGB1 were detected by both Western blot and immunohistochemistry. Serum creatinine (Cr) contents were measured by automatic biochemistry analyzer. In addition, pathological lesions in kidney were observed under light microscope using HE staining.
Compared with sham scald group, both mRNA and protein expressions of HMGB1 were significantly enhanced in the kidney at 8, 24, and 72 hours after scald injury (P<0.05, P<0.01), meanwhile serum Cr contents were markedly increased following acute insults (P<0.05, P<0.01). Treatment with Xuebijing injection could markedly down-regulated renal HMGB1 mRNA expression and protein release at 24 hours and 72 hours (P<0.05, P<0.01), and significantly reduced serum Cr content following scald injury (P<0.05). Many inflammatory cells in renal tissues were observed using light microscope following scald. The histological morphology of kidney lesions was a-HMGB1, a late mediator, appears to be inmeliorated after treatment with Xuebijing injection.
volved in the pathogenesis of excessive inflammatory response and acute kidney damage. Treatment with Xuebijing injection can inhibit HMGB1 synthesis and release in renal tissues, and may prevent the development of acute kidney injury induced by serious scald injury.
探讨烫伤大鼠肾高迁移率族蛋白B1（HMGB1）水平的变化，以及中药血必净注射液对HMGB1表达及急性肾损伤的影响。
将Wistar大鼠行30%全层烫伤后延迟复苏。78只动物分为假烫伤组（n = 18）、烫伤组（n = 30）和血必净注射液治疗组（n = 30）。于烧伤后8、24和72小时处死所有动物，采集肾组织和血液样本，检测HMGB1 mRNA及蛋白表达和器官功能参数。以GAPDH为内参，采用逆转录聚合酶链反应半定量检测HMGB1 mRNA水平，采用蛋白质印迹法和免疫组织化学法检测HMGB1蛋白表达。用自动生化分析仪检测血清肌酐（Cr）含量。此外，用HE染色在光镜下观察肾脏病理损伤。
与假烫伤组相比，烫伤后8、24和72小时肾组织中HMGB1的mRNA和蛋白表达均显著增强（P < \alpha05，P < \alpha01），同时急性损伤后血清Cr含量明显升高（P < \alpha05，P < \alpha01）。血必净注射液治疗可在24小时和72小时显著下调肾组织中HMGB1 mRNA表达和蛋白释放（P < \alpha05，P < \alpha01），并显著降低烫伤后血清Cr含量（P < \alpha05）。光镜下观察烫伤后肾组织中有许多炎性细胞。血必净注射液治疗后，肾脏损伤的组织形态学得到改善。晚期介质α - HMGB1似乎参与了过度炎症反应和急性肾损伤的发病机制。
血必净注射液治疗可抑制肾组织中HMGB1的合成和释放，并可能预防严重烫伤所致急性肾损伤的发生。
