Mottaghy K, Oedekoven B, Pöppel K, Bruchmüller K, Kovacs B, Spahn A, Geisen C
Department of Physiology, Technical University of Aachen, West Germany.
ASAIO Trans. 1989 Jul-Sep;35(3):635-7. doi: 10.1097/00002480-198907000-00152.
As already shown, a single bolus injection of heparin is sufficient to perform extracorporeal circulation (ECC) for long-term lung support if bioactive surfaces (HBS) are used. In the present study whether complete avoidance of systemic heparinization using HBS is possible was studied. Seven conscious sheep (50-60 kg) underwent venovenous bypass under standardized conditions using a Maxima capillary membrane oxygenator. Drug administration, surgery, and other conditions, such as priming volume and blood flow rates, were identical in all experiments. All blood contacting surfaces (oxygenator, catheters, etc.) were HBS. The ECC periods were set for 24 hr (n = 3) and 5 days (n = 4). At no time was heparin administered. No complications occurred in any experiment. Partial thromboplastin time, activated clotting time, and other coagulation parameters remained within physiologic ranges. Platelets did not drop below 80% of baseline values, and hemolysis rates were negligible. Blood gas data and other vital parameters were normal during the entire ECC period. Results show that HBS can be applied to long-term ECC, without the necessity for heparin administration.
如前所示，如果使用生物活性表面（HBS），单次推注肝素就足以进行体外循环（ECC）以实现长期肺支持。在本研究中，探讨了使用HBS是否能够完全避免全身肝素化。七只清醒的绵羊（体重50 - 60千克）在标准化条件下使用Maxima毛细管膜氧合器进行静脉 - 静脉旁路。在所有实验中，药物给药、手术以及其他条件，如预充量和血流速率均相同。所有血液接触表面（氧合器、导管等）均为HBS。ECC时间设定为24小时（n = 3）和5天（n = 4）。在任何时候都未给予肝素。在任何实验中均未发生并发症。部分凝血活酶时间、活化凝血时间和其他凝血参数均保持在生理范围内。血小板未降至基线值的80%以下，溶血率可忽略不计。在整个ECC期间，血气数据和其他生命参数均正常。结果表明，HBS可应用于长期ECC，无需给予肝素。
