State Key Laboratory of Dairy Biotechnology, Shanghai 200436, China.
Shanghai Engineering Research Center of Dairy Biotechnology, China.
Carbohydr Polym. 2014 Nov 4;112:556-62. doi: 10.1016/j.carbpol.2014.06.035. Epub 2014 Jun 20.
The characteristics of the growth of Leuconostoc mesenteroides BD1710 and the synthesis of dextran in tomato juice supplemented with 15% sucrose were assayed. L. mesenteroides BD1710 could synthesize approximately 32 g L(-1) dextran in the tomato-juice-sucrose medium when cultured at 28 °C for 48 h, which was on the same level as the dextran yield in a chemically defined medium. The viscosity of the cultured tomato-juice-sucrose medium with various dextran contents was also measured. The results of the monosaccharide composition, molecular-weight distribution, Fourier transform infrared spectra (FTIR) and nuclear magnetic resonance spectra (NMR) showed that the polysaccharide synthesized by L. mesenteroides BD1710 in the tomato-juice-sucrose medium was dextran with a peak molecular weight of 6.35 × 10(5)Da, a linear backbone composed of consecutive α-(1 → 6)-linked d-glucopyranosyl units and approximately 6% α-(1 → 3) branches.
研究了 Leuconostoc mesenteroides BD1710 的生长特性和在添加 15%蔗糖的番茄汁中合成葡聚糖的情况。当在 28°C 下培养 48 小时时，L. mesenteroides BD1710 可以在番茄汁-蔗糖培养基中合成约 32 g/L 的葡聚糖，与化学定义培养基中的葡聚糖产量相当。还测量了具有不同葡聚糖含量的培养的番茄汁-蔗糖培养基的粘度。单糖组成、分子量分布、傅里叶变换红外光谱 (FTIR) 和核磁共振光谱 (NMR) 的结果表明，L. mesenteroides BD1710 在番茄汁-蔗糖培养基中合成的多糖是葡聚糖，其峰值分子量为 6.35×10(5)Da，线性主链由连续的 α-(1→6)-连接的 d-吡喃葡萄糖基单元组成，并且有约 6%的 α-(1→3)支链。
