Nakajima Yuki, Hattori Tetsuya, Asano Atsushi, Ishikawa Naoto, Tajima Atsushi
Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan; Fellow of the Japanese Society for the Promotion of Science.
J Reprod Dev. 2014;60(6):406-10. doi: 10.1262/jrd.2013-108. Epub 2014 Aug 16.
A series of experiments was conducted to investigate migration, proliferation and differentiation of gonadal germ cells (GGCs) collected from the gonads of 7-day-old chick embryos under cross-sex germline chimera conditions. The migratory and proliferative abilities of exogenous GGCs were examined by transferring 50 fluorescently labeled GGCs collected from White Leghorn (WL) embryos into the blood of 2-day-old Rhode Island Red (RIR) embryos. No significant difference was observed in the number of fluorescently labeled GGCs in the gonads of recipient embryos among any of the four possible donor and recipient sex combinations. Cross-sex germline chimeras were produced to examine the differentiation of GGCs by transferring 100 GGCs from WL embryos into 2-day-old RIR embryos. Exogenous-GGC-derived progeny were obtained from both male and female recipients, except when female GGCs were transferred into male recipients. The migratory ability of GGCs recovered from the 7-day-old embryonic gonad was not influenced by cross-sex germ cell transfer conditions, whereas the differentiation of the GGCs was affected by the sex combinations of GGCs donors and recipients.
进行了一系列实验，以研究在跨性别生殖系嵌合体条件下，从7日龄鸡胚性腺中收集的生殖腺生殖细胞（GGCs）的迁移、增殖和分化情况。通过将从白来航（WL）胚胎中收集的50个荧光标记的GGCs转移到2日龄罗德岛红（RIR）胚胎的血液中，检测外源性GGCs的迁移和增殖能力。在四种可能的供体和受体性别组合中，受体胚胎性腺中荧光标记的GGCs数量均未观察到显著差异。通过将100个来自WL胚胎的GGCs转移到2日龄RIR胚胎中，产生跨性别生殖系嵌合体，以检测GGCs的分化情况。除了将雌性GGCs转移到雄性受体中之外，在雄性和雌性受体中均获得了外源性GGCs衍生的后代。从7日龄胚胎性腺中回收的GGCs的迁移能力不受跨性别生殖细胞转移条件的影响，而GGCs的分化则受GGCs供体和受体的性别组合影响。
