Sugimoto T, Takemura M, Ichikawa H, Akai M
2nd Department of Oral Anatomy, Osaka University Faculty of Dentistry, Japan.
Brain Res. 1989 Dec 29;505(2):354-7. doi: 10.1016/0006-8993(89)91468-6.
Trigeminal primary afferent neuronal cell bodies were labeled with FITC-WGA retrogradely transported from the mandibular molar tooth pulp (tooth pulp) and the cutaneous branch of the mylohyoid nerve (cutaneous nerve) in the rat. Tissue sections were then incubated in Hansson's medium for histological demonstration of carbonic anhydrase (CA) activity. About 85% of primary neurons innervating the tooth pulp had medium to large cell bodies (greater than or equal to 300 microns 2 in cross-sectional area), while 77% of those innervating the cutaneous nerve were small (less than 300 microns 2). A total of 32% of the tooth pulp cells exhibited CA activity. CA-containing cells constituted only about 4% of the cutaneous nerve cells. In view of the known distribution of CA within the rodent nervous system, the CA-containing cells are considered to represent the tooth pulp primaries conducting in A-beta velocity range and projecting to rostral subdivisions of the brainstem sensory trigeminal nuclear complex.
在大鼠中，用从下颌磨牙牙髓（牙髓）和下颌舌骨神经的皮支（皮神经）逆行运输的FITC-WGA标记三叉神经初级传入神经元细胞体。然后将组织切片在汉森培养基中孵育，用于组织学显示碳酸酐酶（CA）活性。支配牙髓的初级神经元中约85%具有中等至大的细胞体（横截面积大于或等于300平方微米），而支配皮神经的初级神经元中77%为小细胞体（小于300平方微米）。总共32%的牙髓细胞表现出CA活性。含CA的细胞仅占皮神经细胞的约4%。鉴于CA在啮齿动物神经系统中的已知分布，含CA的细胞被认为代表以A-β速度范围传导并投射到脑干感觉三叉神经核复合体 Rostral 亚区的牙髓初级神经元。
