Nathanson J A, Kantham L, Hunnicutt E J
Dept. of Neurology, Harvard Medical School, Massachusetts General Hospital, Boston 02114.
FEBS Lett. 1989 Dec 18;259(1):117-20. doi: 10.1016/0014-5793(89)81508-x.
An octopamine receptor photoaffinity probe was used to label membranes from the light organs of Photinus pyralis, a tissue highly enriched in octopamine receptors. Labeling was concentrated in a glycoprotein of 75 +/- 2 kDa with lesser labeling of a 79 +/- 2 kDa component. Labeling could be displaced by prior incubation with octopamine, mianserin, cyproheptadine, phentolamine or propranolol, with a relative potency that correlated with the ability of these same agents to modulate light organ octopamine-sensitive adenylate cyclase. The 75 kDa binding protein was isolated and its N-terminal amino acid sequence was determined.
使用一种章鱼胺受体光亲和探针标记了萤火虫（Photinus pyralis）发光器官的膜，该组织富含章鱼胺受体。标记集中在一种75±2 kDa的糖蛋白上，而79±2 kDa组分的标记较少。预先用章鱼胺、米安色林、赛庚啶、酚妥拉明或普萘洛尔孵育后，标记可被取代，其相对效力与这些相同药物调节发光器官章鱼胺敏感腺苷酸环化酶的能力相关。分离出75 kDa的结合蛋白并测定了其N端氨基酸序列。
