Kawai S, Takahashi K, Kawamoto S, Nagahara A, Sonoda M, Kurosawa T, Onuma M
Nihon Juigaku Zasshi. 1989 Dec;51(6):1219-25. doi: 10.1292/jvms1939.51.1219.
The purpose of this study is to elucidate some morphological characteristics of the bar-structure in bovine erythrocytes infected with Theileria sergenti. The bar-structure, the veil or the both were observed in infected erythrocytes. Infected bovine erythrocytes were classified into four types according to the included structures. Infected cells containing bar-structures increased with the progress of parasitemia. In Giemsa-stained blood preparations, bar-structures appeared purplish-red and measured a mean of 1.6 micron in length and up to 0.1 micron in width. Bar-structures were usually straight, sometimes S-shaped, and located in the periphery of infected erythrocytes. In the direct fluorescent antibody test with T. sergenti-positive bovine serum both piroplasms and bar-structures exhibited fluorescence. However, in the indirect fluorescent antibody test with monoclonal antibodies against piroplasms only piroplasms showed a highly specific fluorescence. Electron microscopy revealed that bar-structures were vesicular in structure, surrounded by a double membrane connected to the host cell membrane.
本研究的目的是阐明感染瑟氏泰勒虫的牛红细胞中杆状结构的一些形态学特征。在感染的红细胞中观察到了杆状结构、面纱样结构或两者皆有。根据所含结构，感染的牛红细胞被分为四种类型。随着寄生虫血症的进展，含有杆状结构的感染细胞数量增加。在吉姆萨染色的血液涂片上，杆状结构呈紫红色，平均长度为1.6微米，宽度可达0.1微米。杆状结构通常是直的，有时呈S形，位于感染红细胞的周边。在用瑟氏泰勒虫阳性牛血清进行的直接荧光抗体试验中，梨形虫和杆状结构均呈现荧光。然而，在用抗梨形虫单克隆抗体进行的间接荧光抗体试验中，只有梨形虫显示出高度特异性荧光。电子显微镜显示，杆状结构呈泡状，被与宿主细胞膜相连的双层膜所包围。
