Turrioni A P S, Basso F G, Alonso J R L, de Oliveira C F, Hebling J, Bagnato V S, de Souza Costa C A
Oper Dent. 2015 Jan-Feb;40(1):102-11. doi: 10.2341/13-370-L. Epub 2014 Aug 19.
The aim of this study was to investigate the effects of transdentinal irradiation with different light-emitting diode (LED) parameters on odontoblast-like cells (MDPC-23).
Human dentin discs (0.2 mm thick) were obtained, and cells were seeded on their pulp surfaces with complete culture medium (Dulbecco modified Eagle medium). Discs were irradiated from the occlusal surfaces with LED at different wavelengths (450, 630, and 840 nm) and energy densities (0, 4, and 25 J/cm(2)). Cell viability (methyltetrazolium assay), alkaline phosphatase activity (ALP), total protein synthesis (TP), and cell morphology (scanning electron microscopy) were evaluated. Gene expression of collagen type I (Col-I) was analyzed by quantitative polymerase chain reaction (PCR). Data were analyzed by the Mann-Whitney test with a 5% significance level.
Higher cell viability (21.8%) occurred when the cells were irradiated with 630 nm LED at 25 J/cm(2). Concerning TP, no statistically significant difference was observed between irradiated and control groups. A significant increase in ALP activity was observed for all tested LED parameters, except for 450 nm at 4 J/cm(2). Quantitative PCR showed a higher expression of Col-I by the cells subjected to infrared LED irradiation at 4 J/cm(2). More attached cells were observed on dentin discs subjected to irradiation at 25 J/cm(2) than at 4 J/cm(2).
The infrared LED irradiation at an energy density of 4 J/cm(2) and red LED at an energy density of 25 J/cm(2) were the most effective parameters for transdentinal photobiomodulation of cultured odontoblast-like cells.
本研究旨在探讨不同发光二极管（LED）参数的经牙本质照射对成牙本质细胞样细胞（MDPC-23）的影响。
获取人牙本质盘（0.2毫米厚），并将细胞接种于其牙髓表面，使用完全培养基（杜氏改良 Eagle 培养基）。用不同波长（450、630 和 840 纳米）和能量密度（0、4 和 25 J/cm²）的 LED 从咬合面照射牙本质盘。评估细胞活力（甲基四氮唑法）、碱性磷酸酶活性（ALP）、总蛋白合成（TP）和细胞形态（扫描电子显微镜）。通过定量聚合酶链反应（PCR）分析 I 型胶原（Col-I）的基因表达。数据采用 Mann-Whitney 检验进行分析，显著性水平为 5%。
当细胞用 630 纳米 LED 以 25 J/cm²照射时，细胞活力更高（21.8%）。关于 TP，照射组和对照组之间未观察到统计学上的显著差异。除了 450 纳米以 4 J/cm²照射外，所有测试的 LED 参数均观察到 ALP 活性显著增加。定量 PCR 显示，以 4 J/cm²接受红外 LED 照射的细胞中 Col-I 的表达更高。与以 4 J/cm²照射的牙本质盘相比，在以 25 J/cm²照射的牙本质盘上观察到更多附着的细胞。
能量密度为 4 J/cm²的红外 LED 照射和能量密度为 25 J/cm²的红色 LED 照射是培养的成牙本质细胞样细胞经牙本质光生物调节的最有效参数。
