豚鼠角质形成细胞分离物凝集素结合的细胞荧光分析研究
Cytofluorometric study on lectin binding of isolated guinea pig keratinocytes.
作者信息
Nomura H, Hachisuka H, Higuchi M, Sasai Y
机构信息
Department of Dermatology, Kurume University School of Medicine, Japan.
出版信息
Acta Histochem. 1989;87(1):59-62. doi: 10.1016/S0065-1281(89)80032-7.
Lectin binding was cytofluorometrically measured on fractionated keratinocytes of guinea pig. Free keratinocytes were obtained by treatment of EDTA and trypsin. After the treatment, they were separated into 3 fractions by centrifugation on a continuous colloidal silica (Percoll) density gradient. Cells in each fraction were stained by biotinyl lectins and avidin-FITC, and fluorescence intensity was measured by cytofluorometry. Results obtained indicate that little cell surface glycoconjugate is lost during the preparation of free keratinocytes.
采用细胞荧光法检测豚鼠角质形成细胞分离组分的凝集素结合情况。通过用乙二胺四乙酸（EDTA）和胰蛋白酶处理获得游离角质形成细胞。处理后，通过在连续胶体二氧化硅（Percoll）密度梯度上离心将其分为3个组分。每个组分中的细胞用生物素化凝集素和抗生物素蛋白 - 异硫氰酸荧光素（avidin - FITC）染色，并用细胞荧光法测量荧光强度。所得结果表明，在游离角质形成细胞的制备过程中，很少有细胞表面糖缀合物丢失。
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