Alvarez R D, Grizzle W E, Smith L J, Miller D M
Department of Obstetrics and Gynecology, University of Alabama, Birmingham 35294.
Am J Obstet Gynecol. 1989 Dec;161(6 Pt 1):1653-7. doi: 10.1016/0002-9378(89)90944-7.
The mechanism by which compensatory ovarian growth occurs is complex and not completely understood. To compare the molecular events in compensatory ovarian growth with those known to occur in other compensatory growth processes such as the regenerating liver, the temporal pattern of proto-oncogene expression and dexoyribonucleic acid synthesis was investigated in rat ovarian tissue after unilateral castration. One hundred fifty female rats were subjected to either a left hemioophorectomy or a sham oophorectomy. Twenty-four rats from each group were put to death at 3 and 14 days after the initial procedure and the ovaries were weighed. There was a mean compensatory weight increase in the right ovaries of the hemioophorectomy group of 7.9% at 3 days and 22.5% at 14 days. The temporal pattern of proto-oncogene expression was determined by removing the right ovary from six rats in each group at 4, 8, 12, 24, 36, and 48 hours after the initial procedure. The ovaries were paired into three samples in each group for each time point and the ribonucleic acid was extracted. Dot blot hybridization was performed on each ribonucleic acid sample with radiolabeled complementary deoxyribonucleic acid probes for the proto-oncogenes c-myc, c-HA-ras, and c-fos. There was no significant increase in proto-oncogene expression in the right ovaries of the hemioophorectomy group when compared with the right ovaries of the sham oophorectomy group. The temporal pattern of dexoyribonucleic acid synthesis was determined by removing the right ovary from three rats in each group at 8, 12, 24, 36, and 48 hours after the initial procedure. Each rat had been injected intraperitoneally with [3H]thymidine 2 hours before the right oophorectomy. The specific activity of dexoyribonucleic acid extracted from each ovarian sample did not demonstrate a significant increase in ovarian dexoyribonucleic acid synthesis after hemioophorectomy or any significant difference in dexoyribonucleic acid synthesis between the hemioophorectomy and the sham oophorectomy groups. This report concludes that compensatory ovarian growth occurs by a mechanism distinct from compensatory growth in the regenerating liver.
代偿性卵巢生长发生的机制很复杂，尚未完全明确。为了比较代偿性卵巢生长过程中的分子事件与已知发生在其他代偿性生长过程（如再生肝脏）中的分子事件，研究人员对单侧卵巢切除术后大鼠卵巢组织中原癌基因表达和脱氧核糖核酸合成的时间模式进行了研究。150只雌性大鼠接受了左侧半卵巢切除术或假卵巢切除术。每组24只大鼠在初始手术后3天和14天处死，并对卵巢称重。半卵巢切除术组右侧卵巢的平均代偿性重量增加在术后3天为7.9%，在术后14天为22.5%。通过在初始手术后4、8、12、24、36和48小时从每组6只大鼠中切除右侧卵巢，来确定原癌基因表达的时间模式。在每个时间点，每组的卵巢被配对成三个样本并提取核糖核酸。用放射性标记的原癌基因c-myc、c-HA-ras和c-fos的互补脱氧核糖核酸探针，对每个核糖核酸样本进行斑点印迹杂交。与假卵巢切除术组的右侧卵巢相比，半卵巢切除术组右侧卵巢中原癌基因表达没有显著增加。通过在初始手术后8、12、24、36和48小时从每组3只大鼠中切除右侧卵巢，来确定脱氧核糖核酸合成的时间模式。在右侧卵巢切除术前2小时，每只大鼠已腹腔注射[3H]胸腺嘧啶核苷。从每个卵巢样本中提取的脱氧核糖核酸的比活性未显示半卵巢切除术后卵巢脱氧核糖核酸合成有显著增加，也未显示半卵巢切除术组和假卵巢切除术组之间在脱氧核糖核酸合成上有任何显著差异。本报告得出结论，代偿性卵巢生长的发生机制与再生肝脏的代偿性生长不同。
