Gonzalez Adriana María Neira, Gutierrez Rosa Martha Perez, Cotera Luis Bernardo Flores
Laboratory of Microbial Secondary Metabolites, Department of Biotechnology and Biengineering, Cinvestav, México DF, 07360, México.
Chin J Integr Med. 2014 Aug 20. doi: 10.1007/s11655-014-1753-2.
To investigate the effect of hypoglycaemic, hypolipidemic, oxidative stress, insulin resistance and advanced glycation endproducts (AGEs) formation of hexane extract from Piper auritum.
The streptozotocin (STZ)-induced diabetic Wistar rats were treated with the hexane extract from Piper auritum leaves for 28 days and a set of biochemical parameters were studied including glucose level, total cholesterol, triglycerides, lipid peroxidation, liver and muscle glycogen, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. The liver function was observed by determining glucose-6-phosphatase, glucokinase and hexokinase activities, and the effect of the hexane extract on insulin level and protein glycation.
There was a significant increase in blood glucose level (P<0.05) in diabetic rats after 24 h of STZ injection. There was a significantly decreased in blood glucose in diabetic rats with hexane extract treatment (P<0.05). The serum biochemical parameters, hepatic enzymes, thiobarbituric acid reactive substances, glycosylated hemoglobin, AGEs, and insulin level (P<0.01 or P<0.05) were restored to normal levels in STZ-diabetic rats treated with hexane extract.
The hexane extract from Piper auritum leaves can efficiently inhibit insulin resistance, AGEs formation, improvement of renal function, lipid abnormalities and oxidative stress, indicating that its therapeutic properties may be due to the interaction of hexane extract components with multiple targets involved in diabetes pathogenesis.
研究墨西哥多香果己烷提取物对血糖、血脂、氧化应激、胰岛素抵抗及晚期糖基化终产物（AGEs）形成的影响。
用墨西哥多香叶己烷提取物对链脲佐菌素（STZ）诱导的糖尿病Wistar大鼠进行为期28天的治疗，并研究一组生化参数，包括血糖水平、总胆固醇、甘油三酯、脂质过氧化、肝糖原和肌糖原、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶。通过测定葡萄糖-6-磷酸酶、葡萄糖激酶和己糖激酶的活性来观察肝功能，以及己烷提取物对胰岛素水平和蛋白质糖基化的影响。
STZ注射24小时后，糖尿病大鼠的血糖水平显著升高（P<0.05）。己烷提取物治疗的糖尿病大鼠血糖显著降低（P<0.05）。用己烷提取物治疗的STZ糖尿病大鼠的血清生化参数、肝酶、硫代巴比妥酸反应物质、糖化血红蛋白、AGEs和胰岛素水平（P<0.01或P<0.05）恢复到正常水平。
墨西哥多香叶己烷提取物可有效抑制胰岛素抵抗、AGEs形成，改善肾功能、脂质异常和氧化应激，表明其治疗特性可能归因于己烷提取物成分与糖尿病发病机制中多个靶点的相互作用。
