印度玉米螟对苏云金芽孢杆菌杀虫蛋白Cry1A.105和Cry2Ab2的基线敏感性

Baseline sensitivity of maize borers in India to the Bacillus thuringiensis insecticidal proteins Cry1A.105 and Cry2Ab2.

作者信息

Jalali Sushil K, Yadavalli Lalitha, Ojha Rakshit, Kumar Pradyumn, Sulaikhabeevi Suby B, Sharma Reema, Nair Rupa, Kadanur Ravi C, Kamath Subray P, Komarlingam Mohan S

机构信息

National Bureau of Agriculturally Important Insects, Hebbal, Bangalore, India.

Directorate of Maize Research, Pusa Campus, New Delhi, India.

出版信息

Pest Manag Sci. 2015 Aug;71(8):1082-90. doi: 10.1002/ps.3888. Epub 2014 Sep 25.

DOI:10.1002/ps.3888

PMID:25143318

Abstract

BACKGROUND

Among the major pests of maize in India are two stem borers, Chilo partellus (Swinhoe) and Sesamia inferens (Walker), and an earworm, Helicoverpa armigera (Hübner). As a pest control strategy, transgenic Bacillus thuringiensis (Bt) maize hybrids are undergoing regulatory trials in India. We have determined the sensitivity of the target lepidopterans to the insecticidal Bt proteins expressed in Bt maize, as this determines product efficacy and the resistance management strategy to be adopted. Maize hybrids with event MON89034 express two insecticidal Bt proteins, Cry1A.105 and Cry2Ab2.

RESULTS

Sensitivity profiles of 53 populations of C. partellus, 21 populations of S. inferens and 21 populations of H. armigera, collected between 2008 and 2013 from maize-growing areas in India, to Cry1A.105 and Cry2Ab2 proteins were generated through dose-response assays. Cry1A.105 protein was the most effective to neonates of C. partellus (mean MIC90 range 0.30-1.0 µg mL(-1) ) and H. armigera (mean MIC90 range 0.71-8.22 µg mL(-1) ), whereas Cry2Ab2 (mean MIC90 range 0.65-1.70 µg mL(-1) ) was the most effective to S. inferens.

CONCLUSION

Populations of C. partellus, S. inferens and H. armigera were susceptible to the Bt proteins Cry1A.105 and Cry2Ab2. The Bt sensitivity data will serve as precommercialisation benchmarks for resistance monitoring purposes.

摘要

背景

在印度，玉米的主要害虫有两种茎螟，即印度谷螟（Chilo partellus，斯温霍）和大螟（Sesamia inferens，沃克），以及一种穗虫，即棉铃虫（Helicoverpa armigera，胡伯纳）。作为一种害虫防治策略，转基因苏云金芽孢杆菌（Bt）玉米杂交种正在印度进行监管试验。我们已经确定了目标鳞翅目昆虫对Bt玉米中表达的杀虫Bt蛋白的敏感性，因为这决定了产品的功效以及要采用的抗性管理策略。具有事件MON89034的玉米杂交种表达两种杀虫Bt蛋白，即Cry1A.105和Cry2Ab2。

结果

通过剂量反应试验，得出了2008年至2013年期间从印度玉米种植区采集的53个印度谷螟种群、21个大螟种群和21个棉铃虫种群对Cry1A.105和Cry2Ab2蛋白的敏感性概况。Cry1A.105蛋白对印度谷螟幼虫（平均MIC90范围为0.30 - 1.0 μg mL(-1)）和棉铃虫幼虫（平均MIC90范围为0.71 - 8.22 μg mL(-1)）最有效，而Cry2Ab2（平均MIC90范围为0.65 - 1.70 μg mL(-1)）对大螟最有效。