Cagirdi Duygu, Altundag Hüseyin, Imamoglu Mustafa, Tuzen Mustafa
J AOAC Int. 2014 Jul-Aug;97(4):1137-42. doi: 10.5740/jaoacint.12-409.
A simple and selective separation and preconcentration method was developed for the determination of Cu(ll) ions. This method is based on adsorption of Cu(ll) ions from aqueous solution on a bis(3-aminopropyl)amine modified silica gel column and flame atomic absorption spectrometric determination after desorption. Various analytical parameters such as pH, type of eluent solution and its volume, flow rate of sample and eluent, and sample volume were optimized. Effects of some cation, anion, and transition metal ions on the recoveries of Cu(ll) ions were also investigated. Cu(ll) ions were quantitatively recovered at pH 6; 5.0 mL of 2 M HCI was used as the eluent. The preconcentration factor was found to be 150. The LOD was 0.12 microg/L for Cu(ll). The accuracy of the method was confirmed by analysis of Tea Leaves (INCT-TL-1) and Fish Protein (DORM-3) certified reference materials. The optimized method was applied to various water and food samples for the determination of Cu(ll).
开发了一种简单且选择性的分离和预富集方法用于测定铜（II）离子。该方法基于将水溶液中的铜（II）离子吸附在双（3-氨丙基）胺修饰的硅胶柱上，然后解吸后用火焰原子吸收光谱法测定。对各种分析参数进行了优化，如pH值、洗脱液类型及其体积、样品和洗脱液的流速以及样品体积。还研究了一些阳离子、阴离子和过渡金属离子对铜（II）离子回收率的影响。在pH 6时可定量回收铜（II）离子；使用5.0 mL 2 M盐酸作为洗脱液。预富集因子为150。铜（II）的检测限为0.12 μg/L。通过分析茶叶（INCT-TL-1）和鱼蛋白（DORM-3）标准参考物质证实了该方法的准确性。将优化后的方法应用于各种水和食品样品中铜（II）的测定。
