Keleti T, Berni R, Vas M, Mozzarelli A, Rossi G L
Institute of Enzymology, Hungarian Academy of Sciences, Budapest.
Acta Biochim Biophys Hung. 1989;24(1-2):15-23.
Pairwise coupled reactions of fructose-1,6-bisphosphate aldolase and sn-glycerol-3-phosphate dehydrogenase, 3-phosphoglycerate kinase and D-glyceraldehyde-3-phosphate dehydrogenase, triosephosphate isomerase and sn-glycerol-3-phosphate dehydrogenase have been detected by microspectrophotometry in single crystals obtained from myogen A in the presence of polyethylene glycol. Microspectrophotometric measurements with polarized light demonstrate that the protein molecules are oriented and that NADH is bound with a definite orientation to the dehydrogenases within the crystal.
在聚乙二醇存在的情况下，通过显微分光光度法在从肌浆A获得的单晶中检测到了果糖-1,6-二磷酸醛缩酶与sn-甘油-3-磷酸脱氢酶、3-磷酸甘油酸激酶与D-甘油醛-3-磷酸脱氢酶、磷酸丙糖异构酶与sn-甘油-3-磷酸脱氢酶的成对偶联反应。用偏振光进行的显微分光光度测量表明，蛋白质分子是定向排列的，并且NADH以确定的方向与晶体内的脱氢酶结合。
