Schultka R, Schmidt T, Hepp W D, Schräpler R, Cech S
Institute für Anatomie, Martin-Luther-Universität Halle-Wittenberg.
Acta Histochem. 1989;86(2):159-66.
The Picrosirius-polarization method was applied to the selective morphological collagen detection in the human uterine tube. Solaminrot 4B (VEB Chemiekombinat Bitterfeld) was used as dyestuff which enhances the normal birefringency of collagen fibrous material in tissue sections. The results show that the connective tissue of the lamina propria mucosae consists of collagen fibres, namely considerably. The mucosal folds of uterine tubes of younger individuals contain delicate, weaker birefringent fibres stained yellowish-red and red-orange. The folds of oviducts of ageing individuals composed of compact, strong birefringent collagen fibrous material stained intensely red, red-orange, and yellow. This is characteristic of organs in the pre- and postmenopausal periods and in the senium. The findings suggest that the fibrous structures detected selectively in the oviductal mucosa by combinative method of Picrosolamin-staining and polarization microscopy are distinct collagen fibres. The delicate, weaker birefringent fibres could correspond with collagen type III and the thicker, stronger birefringent ones could represent collagen type I.
采用天狼星红偏振光法对人输卵管进行选择性形态学胶原检测。使用索拉明罗特4B(德国比特费尔德化学联合企业)作为染料,该染料可增强组织切片中胶原纤维材料的正常双折射。结果表明,固有层黏膜的结缔组织主要由胶原纤维组成。年轻个体输卵管的黏膜皱襞含有纤细、双折射较弱的纤维,染成黄红色和红橙色。老年个体输卵管的皱襞由紧密、双折射强的胶原纤维材料组成,染成深红色、红橙色和黄色。这是绝经前、绝经后及老年期器官的特征。研究结果表明,通过索拉明染色和偏振显微镜联合方法在输卵管黏膜中选择性检测到的纤维结构是不同的胶原纤维。纤细、双折射较弱的纤维可能对应Ⅲ型胶原,而较粗、双折射较强的纤维可能代表Ⅰ型胶原。