2'-脱氧烟酰胺用于ADP-核糖转移反应。

Utilization of 2'-deoxynad for ADP-ribose transfer reactions.

作者信息

Wasson D B, Yamanaka H, Carson D A

机构信息

Department of Basic and Clinical Research, Scripps Clinic & Research Foundation, La Jolla, California.

出版信息

Adv Exp Med Biol. 1989;253B:213-8. doi: 10.1007/978-1-4684-5676-9_32.

DOI:10.1007/978-1-4684-5676-9_32

PMID:2514587

Abstract

2'-deoxyNAD was examined as a substrate for both mono(ADP-ribosyl)ation and poly(ADP-ribosyl)ation reactions. 2'-deoxyNAD is a substrate for the diphtheria toxin-catalyzed mono(ADP-ribosyl)ation of elongation factor-2, inactivating its function to enhance protein synthesis. On the other hand, 2'-deoxyNAD is a poor substrate for poly(ADP-ribose) polymerase. 2'-deoxyNAD was not synthesized intracellularly from deoxyATP, even when deoxyATP content was markedly increased by incubation of cells with deoxyadenosine and an adenosine deaminase inhibitor. 2'-deoxyNAD, because of its specificity, could be a quite useful reagent for the investigation of cellular mono(ADP-ribosyl)ation reactions.

摘要

2'-脱氧烟酰胺腺嘌呤二核苷酸（2'-deoxyNAD）被作为单（ADP-核糖基）化反应和多（ADP-核糖基）化反应的底物进行了研究。2'-脱氧NAD是白喉毒素催化的延伸因子-2单（ADP-核糖基）化反应的底物，可使其增强蛋白质合成的功能失活。另一方面，2'-脱氧NAD是聚（ADP-核糖）聚合酶的不良底物。即使通过用脱氧腺苷和腺苷脱氨酶抑制剂孵育细胞使脱氧ATP含量显著增加，细胞内也不会由脱氧ATP合成2'-脱氧NAD。由于其特异性，2'-脱氧NAD可能是研究细胞单（ADP-核糖基）化反应的非常有用的试剂。