Fang Zheng-wu, Qi Rui, Li Xiao-fang, Liu Zhi-xiong
Institute of Crop Genetics and Breeding, Yangtze University, Jingzhou City, Hubei 434025, PR China.
College of Horticulture and Gardening, Yangtze University, Jingzhou City, Hubei 434025, PR China.
Gene. 2014 Oct 25;550(2):200-6. doi: 10.1016/j.gene.2014.08.029. Epub 2014 Aug 19.
Arabidopsis thaliana APETALA3 (AP3) and Antirrhinum majus DEFICIENS (DEF) MADS box genes are required to specify petal and stamen identity. AP3 and DEF are members of the euAP3 lineage, which arose by gene duplication coincident with radiation of the core eudicots. In order to investigate the molecular mechanisms underlying organ development in early diverging clades of core eudicots, we isolated and identified an AP3 homolog, FaesAP3, from Fagopyrum esculentum (buckwheat, Polygonaceae), a multi-food-use pseudocereal with healing benefits. Protein sequence alignment and phylogenetic analyses revealed that FaesAP3 grouped into the euAP3 lineage. Expression analysis showed that FaesAP3 was transcribed only in developing stamens, and differed from AP3 and DEF, which expressed in developing petals and stamens. Moreover, ectopic expression of FaesAP3 rescued stamen development without complementation of petal development in an Arabidopsis ap3 mutant. Our results suggest that FaesAP3 is involved in the development of stamens in buckwheat. These results also suggest that FaesAP3 holds some potential for biotechnical engineering to create a male sterile line of F. esculentum.
拟南芥APETALA3（AP3）和金鱼草DEFICIENS（DEF）MADS盒基因对于确定花瓣和雄蕊的特性是必需的。AP3和DEF是euAP3谱系的成员，该谱系通过与核心真双子叶植物辐射同时发生的基因复制而产生。为了研究核心真双子叶植物早期分化分支中器官发育的分子机制，我们从具有药用价值的多用途伪谷物荞麦（蓼科）中分离并鉴定了一个AP3同源基因FaesAP3。蛋白质序列比对和系统发育分析表明，FaesAP3归入euAP3谱系。表达分析表明，FaesAP3仅在发育中的雄蕊中转录，这与在发育中的花瓣和雄蕊中表达的AP3和DEF不同。此外，在拟南芥ap3突变体中，FaesAP3的异位表达挽救了雄蕊发育，但未补充花瓣发育。我们的结果表明，FaesAP3参与了荞麦雄蕊的发育。这些结果还表明，FaesAP3在生物技术工程中具有创造荞麦雄性不育系的潜力。
