Falcone Franco H, Gibbs Bernhard F
Division of Molecular and Cellular Science, The School of Pharmacy, The University of Nottingham, Boots Science Building, Science Road, Nottingham, NG7 2RD, UK,
Methods Mol Biol. 2014;1192:35-47. doi: 10.1007/978-1-4939-1173-8_2.
The purification of basophils from peripheral blood has represented a formidable challenge for researchers since they were discovered by Paul Ehrlich in 1879. From the first published attempts in the late 1960s, it took half a century to develop robust protocols able to provide sufficient numbers of pure, functionally unimpaired basophils. The existing protocols for basophil purification exploit those properties of basophils which distinguish them from other cell types such as their localization in blood, density, and the presence or absence of surface markers. Purification techniques have been used in various combinations and variations to achieve a common goal in mind: to obtain a pure population of human basophils in sufficient numbers for downstream studies. The arduous way leading up to the modern protocols is summarized in this historical retrospective. A fast protocol for purification of basophils to near homogeneity is also described.
自1879年保罗·埃尔利希发现嗜碱性粒细胞以来，从外周血中纯化嗜碱性粒细胞一直是研究人员面临的一项艰巨挑战。从20世纪60年代末首次发表的尝试开始，花了半个世纪才开发出强大的方案，能够提供足够数量的纯净、功能未受损的嗜碱性粒细胞。现有的嗜碱性粒细胞纯化方案利用了嗜碱性粒细胞与其他细胞类型不同的特性，例如它们在血液中的定位、密度以及表面标志物的有无。纯化技术以各种组合和变体形式使用，以实现一个共同目标：获得足够数量的纯净人类嗜碱性粒细胞群体用于下游研究。这篇历史回顾总结了通向现代方案的艰难历程。还描述了一种将嗜碱性粒细胞纯化至接近同质性的快速方案。
