测量嗜碱性粒细胞和肥大细胞释放组胺和细胞因子的情况。

Measuring histamine and cytokine release from basophils and mast cells.

作者信息

Jensen Bettina M, Falkencrone Sidsel, Skov Per S

机构信息

Allergy Clinic, Dept. 22, 1. floor, Gentofte Hospital, Niels Andersens vej 65, 2900, Hellerup, Denmark,

出版信息

Methods Mol Biol. 2014;1192:135-45. doi: 10.1007/978-1-4939-1173-8_10.

DOI:10.1007/978-1-4939-1173-8_10

PMID:25149489

Abstract

Basophils and mast cells are known for their capability to release both preformed and newly synthesized inflammatory mediators. In this chapter we describe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detection.

摘要

嗜碱性粒细胞和肥大细胞以其释放预先形成的和新合成的炎症介质的能力而闻名。在本章中，我们描述了如何刺激和检测全血中的嗜碱性粒细胞、纯化的嗜碱性粒细胞、体外培养的肥大细胞以及原位皮肤肥大细胞释放的组胺。我们还给出了一个嗜碱性粒细胞和肥大细胞细胞因子释放的激活方案示例，并讨论了细胞因子检测方法。