嗜酸硫化芽孢杆菌新鉴定出的耐热酯酶：邻苯二甲酸酯降解特性及性能
Newly identified thermostable esterase from Sulfobacillus acidophilus: properties and performance in phthalate ester degradation.
作者信息
Zhang Xiao-Yan, Fan Xiang, Qiu Yong-Jun, Li Cheng-Yuan, Xing Shuai, Zheng Yi-Tao, Xu Jian-He
机构信息
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, Shanghai, China.
出版信息
Appl Environ Microbiol. 2014 Nov;80(22):6870-8. doi: 10.1128/AEM.02072-14. Epub 2014 Aug 22.
EstS1, a newly identified thermostable esterase from Sulfobacillus acidophilus DSM10332, was heterologously expressed in Escherichia coli and shown to enzymatically degrade phthalate esters (PAEs) to their corresponding monoalkyl PAEs. The optimal pH and temperature of the esterase were found to be 8.0 and 70°C, respectively. The half-life of EstS1 at 60°C was 15 h, indicating that the enzyme had good thermostability. The specificity constant (kcat/Km) of the enzyme for p-nitrophenyl butyrate was as high as 6,770 mM(-1) s(-1). The potential value of EstS1 was demonstrated by its ability to effectively hydrolyze 35 to 82% of PAEs (10 mM) within 2 min at 37°C, with all substrates being completely degraded within 24 h. At 60°C, the time required for complete hydrolysis of most PAEs was reduced by half. To our knowledge, this enzyme is a new esterase identified from thermophiles that is able to degrade various PAEs at high temperatures.
EstS1是一种新发现的来自嗜酸硫化芽孢杆菌DSM10332的耐热酯酶，它在大肠杆菌中实现了异源表达，并被证明能将邻苯二甲酸酯（PAEs）酶解为相应的单烷基PAEs。该酯酶的最适pH值和温度分别为8.0和70°C。EstS1在60°C下的半衰期为15小时，表明该酶具有良好的热稳定性。该酶对丁酸对硝基苯酯的特异性常数（kcat/Km）高达6770 mM⁻¹ s⁻¹。EstS1的潜在价值体现在其能够在37°C下2分钟内有效水解35%至82%的PAEs（10 mM），且所有底物在24小时内完全降解。在60°C时，大多数PAEs完全水解所需的时间减半。据我们所知，这种酶是从嗜热菌中鉴定出的一种新型酯酶，能够在高温下降解各种PAEs。
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