Skorvaga M, Nikitina E, Kolenova A, Puskacova J, Leitnerova M, Copakova L, Belyaev Igor
Neoplasma. 2014;61(6):758-65. doi: 10.4149/neo_2014_092.
The precise diagnosis of acute lymphoblastic leukemia is essential for correct prognosis assessment and therapy regimen selection. At present, immunophenotyping, cytogenetics and molecular screening are major and complementary methods utilized in a routine leukemia diagnostics. The aim of this study was to validate the application of multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay for molecular diagnosis of the most common pediatric acute lymphoblastic leukemia-associated fusion transcripts. Our data show that screening of bone marrow and/or peripheral blood by RT-PCR, consisting of multiplex and monoplex PCR, confirmed results of real-time quantitative PCR (RT qPCR). This screening may provide a reliable, specific and sensitive method amenable to standard laboratory practice and a cost-effective alternative to more complex and expensive RT qPCR techniques.
急性淋巴细胞白血病的精确诊断对于正确的预后评估和治疗方案选择至关重要。目前，免疫表型分析、细胞遗传学和分子筛查是常规白血病诊断中使用的主要且互补的方法。本研究的目的是验证多重逆转录 - 聚合酶链反应（RT-PCR）检测法在最常见的儿童急性淋巴细胞白血病相关融合转录本分子诊断中的应用。我们的数据表明，通过由多重和单重PCR组成的RT-PCR对骨髓和/或外周血进行筛查，证实了实时定量PCR（RT qPCR）的结果。这种筛查可能提供一种可靠、特异且灵敏的方法，适用于标准实验室操作，并且是一种比更复杂且昂贵的RT qPCR技术更具成本效益的替代方法。
