培养基中某些必需氨基酸缺乏对胰岛素作用于大鼠原代培养肝细胞的影响。在某些必需氨基酸缺乏的培养基中，肝细胞对胰岛素无反应。

Effect of some essential amino acid deficiency in the medium on the action of insulin on primary cultured hepatocytes of rats. Hepatocytes do not respond to insulin in some essential amino acid-deficient medium.

Takenaka A, Ohishi Y, Noguchi T, Naito H

Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.

Int J Biochem. 1989;21(11):1255-63. doi: 10.1016/0020-711x(89)90012-8.

Abstract

The effects of insulin, glucagon and dexamethasone on the amino acid consumption by primary cultures of rat hepatocytes were studied in a medium containing all essential amino acids or in those deficient in some essential or nonessential amino acids. 2. The cells which were cultured in a medium containing all the essential amino acids responded to insulin by enhancing the consumption of amino acids and augmenting protein synthesis. 3. However, the cells did not respond to insulin significantly when they were cultured in a medium deficient in lysine or some other essential amino acids. 4. The results suggest that some essential amino acid deficiency impairs the transmission of the signal of insulin to the site of the metabolic changes induced by the hormone.

摘要

在含有所有必需氨基酸的培养基或缺乏某些必需或非必需氨基酸的培养基中，研究了胰岛素、胰高血糖素和地塞米松对原代培养大鼠肝细胞氨基酸消耗的影响。2. 在含有所有必需氨基酸的培养基中培养的细胞，通过增加氨基酸消耗和增强蛋白质合成来对胰岛素作出反应。3. 然而，当细胞在缺乏赖氨酸或其他一些必需氨基酸的培养基中培养时，它们对胰岛素没有明显反应。4. 结果表明，某些必需氨基酸的缺乏会损害胰岛素信号向该激素诱导的代谢变化部位的传递。